April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Vitrified Collagen Gels with High Optical Transparency and Mechanical Strength for Ocular Repair
Xiomara Calderon-Colon; Jennifer L. Breidenich; Qiongyu Guo; Dan Mulreany; Russell L. McCally; Manny 0. Uy; Jennifer Elisseeff; Jeffrey P. Maranchi; Oliver Schein; Morgana M. Trexler
Author Affiliations & Notes
  • Xiomara Calderon-Colon
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Jennifer L. Breidenich
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Qiongyu Guo
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Dan Mulreany
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University, Baltimore, Maryland
  • Russell L. McCally
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
    Department of Ophthalmology,
    Johns Hopkins University, Baltimore, Maryland
  • Manny 0. Uy
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Jennifer Elisseeff
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Department of Ophthalmology,
    Johns Hopkins University, Baltimore, Maryland
  • Jeffrey P. Maranchi
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Oliver Schein
    Department of Ophthalmology,
    Johns Hopkins University, Baltimore, Maryland
  • Morgana M. Trexler
    Milton Eisenhower Research Center, Department of Biomedical Engineering,
    Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland
  • Footnotes
    Commercial Relationships  Xiomara Calderon-Colon, Johns Hopkins University Applied Physics Laboratory (P); Jennifer L. Breidenich, ohns Hopkins University Applied Physics Laboratory (P); Qiongyu Guo, Johns Hopkins University (P); Dan Mulreany, Johns Hopkins University (P); Russell L. McCally, Johns Hopkins University Applied Physics Laboratory (P); Manny 0. Uy, None; Jennifer Elisseeff, Johns Hopkins University (P); Jeffrey P. Maranchi, Johns Hopkins University Applied Physics Laboratory (P); Oliver Schein, Johns Hopkins University (P); Morgana M. Trexler, Johns Hopkins University Applied Physics Laboratory (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6494. doi:
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      Xiomara Calderon-Colon, Jennifer L. Breidenich, Qiongyu Guo, Dan Mulreany, Russell L. McCally, Manny 0. Uy, Jennifer Elisseeff, Jeffrey P. Maranchi, Oliver Schein, Morgana M. Trexler; Vitrified Collagen Gels with High Optical Transparency and Mechanical Strength for Ocular Repair. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6494.

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Abstract

Purpose: : To optimize the mechanical and optical properties of collagen vitrigels for ocular repair.

Methods: : Vitrigel preparation entails three main stages: mixing, gelation, and vitrification. Equal volumes of culture medium (Fetal Bovine Serum, 20 mM HEPES buffer in DMEM (Dulbecco’s Modified Eagle Medium)) and 0.5% acid collagen solution are uniformly mixed. Gelation is initiated via incubation at 37 °C. During vitrification, time, temperature, and humidity are controlled. Following vitrification, the material is rehydrated with Phosphate Buffered Saline. Four vitrification times (0.5, 1, 2, and 5 weeks), 3 temperatures (5, 15, and 40°C), and 3 humidity levels (20, 40, and 60 %RH) were explored using a design of experiments and correlated with resulting optical transparency and mechanical properties.

Results: : Tailoring the synthesis parameters (time, temperature, and humidity) facilitates control of the transparency and mechanical strength of the biomaterial. Transparency is maximized (91 ± 9 % at 550 nm) via vitrification for 4.2 weeks at 21 °C and 20 % RH. For transparency, the most influential synthesis parameters are the quadratic and two-factor interactions of time*time, followed by temperature*temperature and temperature*humidity. Longer vitrification time yields higher strength and stiffness in preliminary mechanical testing.

Conclusions: : Collagen vitrigels with optimized material properties can be synthesized by systematically controlling vitrification time, temperature, and humidity. These materials show promise for complex ocular reconstruction due to their biocompatibility, biodegradability, transparency and strength.

Keywords: contact lens • cornea: basic science 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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