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Yoshie Itou, Nobuhisa Mizuki, Eiichi Okada; Comparison of Three Contact Lens Care Regimens in Removal of Albumin and Lysozyme from Two Orthokeratology Lenses. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6513.
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© ARVO (1962-2015); The Authors (2016-present)
To compare three types of contact lens care (multipurpose solution, MPS; hydrogen peroxide solution, HPS; and povidone iodine solution, PIS) in the efficiency of albumin and lysozyme removal from orthokeratology lenses under experimental conditions.
Emerald Lens (oprifocon A, fluorosilicone acrylate-based lens; Euclid Systems Corporation, VA) or Alfa Ortho-K Lens (fluorosilicone methacrylate-based lens; Alpha Corporation, Japan) were incubated in either protein-containing saline or saline (sham) for 15 hours at 36 degrees celcius. Protein concentrations were 0.54 mg/ml for albumin and 1.37 mg/ml for lysozyme, which represent concentrations in tear fluid after rigid lens wear. After incubation, lenses were either rinsed with saline (control) or washed by lens care solutions (MPS: Menicon O2 Care Milfa fresh, Menicon Corporation, Japan; HPS: AOSEPT CLEAR CARE, CIBA VISION Corporation, Japan; PIS: Bioclen First Care CT, Ophtecs Corporation, Japan) according to the manufacturer’s instructions. Then, lenses were soaked in 1% sodium dodecyl sulfate, and protein was removed by sonication and vortex mixing. Protein concentration was quantified by Micro BCA Protein Assay Kit (ThermoScientific corporation). All statistical analysis was performed by Steel-Dwass multiple comparison test.
Quantity of albumin deposits was higher (p<0.01) in fluorosilicone acrylate lenses (1.62 µg/lens) compared to fluorosilicone methacrylate lenses (0.49 µg/lens). There was no significance in lysozyme attachment between both lenses (fluorosilicone acrylate 1.86 µg/lens and fluorosilicone methacrylate 1.30 µg/lens; p>0.05). For fluorosilicone acrylate-based lens, the percentage of protein removal from lysozyme-deposited were MPS 100%, HPS 97.9%, PIS 94.0%, and from albumin-deposited lenses, MPS 95.3%, HPS 96.0%, PIS 97.5% compared to control lens. In fluorosilicone methacrylate-based lens the percentage of protein removal from lysozyme-deposited lenses were MPS 90.0%, HPS 72.5%, PIS 85.5%, and from albumin-deposited lenses, MPS 88.6%, HPS 100%, PIS 88.3% compared to control lens. All care regimens significantly removed albumin and lysozyme from lenses compared to control (p<0.01), and there was no significance between protein care regimens (p>0.05).
Multipurpose solution, hydrogen peroxide solution, povidone iodine solution showed equivalent efficacy for albumin and lysozyme removal from two types of orthokeratology lenses.
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