April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Early Effects of Contact Lens Wear on Immune Cell Density of the Ocular Surface: Preliminary Results of a Laser In Vivo Confocal Microscopy Study
Author Affiliations & Notes
  • Yureeda Qazi
    Ophthalmology, Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Andrea Cruzat
    Ophthalmology, Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Neda Baniasadi
    Ophthalmology, Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Lixin Zheng
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Deborah Witkin
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Douglas B. Critser
    Ophthalmology, Contact Lens Service, University of Iowa, Iowa City, Iowa
  • Amy Watts
    Ophthalmology, Contact Lens Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
  • Jill Beyer
    Ophthalmology, Contact Lens Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
  • Christine W. Sindt
    Ophthalmology, Contact Lens Service, University of Iowa, Iowa City, Iowa
  • Pedram Hamrah
    Ophthalmology, Cornea Service, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts
    Ocular Surface and Imaging Center, Massachusetts Eye and Ear Infirmary, Massachusetts
  • Footnotes
    Commercial Relationships  Yureeda Qazi, None; Andrea Cruzat, None; Neda Baniasadi, None; Lixin Zheng, None; Deborah Witkin, None; Douglas B. Critser, None; Amy Watts, Alcon Research Limited (F); Jill Beyer, Alcon Research Limited (F); Christine W. Sindt, Alcon Research Limited (F); Pedram Hamrah, Alcon Research Limited (F)
  • Footnotes
    Support  NIH K08-EY020575, New England Corneal Transplant Research Fund, Falk Medical Research Trust to P.H.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6551. doi:
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      Yureeda Qazi, Andrea Cruzat, Neda Baniasadi, Lixin Zheng, Deborah Witkin, Douglas B. Critser, Amy Watts, Jill Beyer, Christine W. Sindt, Pedram Hamrah; Early Effects of Contact Lens Wear on Immune Cell Density of the Ocular Surface: Preliminary Results of a Laser In Vivo Confocal Microscopy Study. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6551.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To assess the subclinical immune response to various contact lens (CL) solutions in naive CL wearers.

Methods: : Multicenter, randomized, double-blinded, clinical trial. Thirty eyes of 15 naïve CL wearers, fitted with silicone hydrogel CL, were enrolled into one of 3 CL solutions groups (OPTI-FREE RepleniSH® (OFR, n=10); Clear Care® (CC, n=8); ReNu MultiPlus® (RM+, n=12). Corneal, limbal, and conjunctival staining, as well as laser in vivo confocal microscopy (IVCM; HRT3/RCM, Heidelberg Engineering, Heidelberg, Germany) of the central and peripheral corneal quadrants were performed at baseline and 1 week post-CL wear. Two masked observers analyzed the images for dendritic cell (DC) and non-dendritic inflammatory cell (IC) density.

Results: : Minimal to no conjunctival or limbal staining was observed in either group. While the CC and RM+ groups showed mild staining of the inferior cornea, no corneal staining was observed in the OFR group. IVCM demonstrated increased DC density in 40-50% of participants in all groups. At week 1, increased DC density was statistically significant between the three groups (p=0.046) for the temporal cornea but not for other areas. The CC group demonstrated the highest DC density (103±69 cells/mm2), with no significant difference between OFR (66±44 cells/mm2) and RM+ (57±24 cells/mm2). Increase in peripheral IC density approached significance (p=0.08) between groups, and was lowest with OFR. No correlation was found between clinical staining and the IVCM parameters.

Conclusions: : IVCM revealed increased immune cell infiltration in all groups at 1 week after CL wear, which was undetected by routine clinical examinations. Our preliminary data suggest that the increase in DC and IC density was partly due to the CL solution. Moreover, our data suggest that routine slit-lamp examination is not sensitive enough to detect subclinical corneal inflammatory changes.

Clinical Trial: : http://www.clinicaltrials.gov NCT 01250925

Keywords: inflammation • imaging/image analysis: clinical • contact lens 
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