March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Ghrelin Is Expressed In The Ciliary Body And Presents A Significant And Sustained Hypotensive Effect In Acute Glaucoma Models
Author Affiliations & Notes
  • Amandio A. Rocha-Sousa
    Physiology and Cardiothoracic Surgery, University of Porto, Porto, Portugal
    Ophthalmology, University of Porto; Faculty of Medicine, Porto, Portugal
  • Marta Tavares-Silva
    Physiology and Cardiothoracic Surgery, University of Porto, Porto, Portugal
  • Paulo Pereira-Silva
    Ophthalmology, University of Porto; Faculty of Medicine, Porto, Portugal
  • Sara Azevedo-Pinto
    Physiology and Cardiothoracic Surgery, University of Porto, Porto, Portugal
  • Joana Rodrigues-Araújo
    Ophthalmology, University of Porto; Faculty of Medicine, Porto, Portugal
  • Sonia Pinho
    Ophthalmology, University of Porto; Faculty of Medicine, Porto, Portugal
  • Adelino Leite-Moreira
    Physiology and Cardiothoracic Surgery, University of Porto, Porto, Portugal
  • Footnotes
    Commercial Relationships  Amandio A. Rocha-Sousa, None; Marta Tavares-Silva, None; Paulo Pereira-Silva, None; Sara Azevedo-Pinto, None; Joana Rodrigues-Araújo, None; Sonia Pinho, None; Adelino Leite-Moreira, None
  • Footnotes
    Support  Grant nº PTDC/SAU-ORG/110683/2009 from the portuguese "Fundação para a Ciência e Tecnologia"
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5110. doi:
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      Amandio A. Rocha-Sousa, Marta Tavares-Silva, Paulo Pereira-Silva, Sara Azevedo-Pinto, Joana Rodrigues-Araújo, Sonia Pinho, Adelino Leite-Moreira; Ghrelin Is Expressed In The Ciliary Body And Presents A Significant And Sustained Hypotensive Effect In Acute Glaucoma Models. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5110.

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Abstract
 
Purpose:
 

To investigate ghrelin’s role in the modulation of IOP in animal models of acute glaucoma and ghrelin’s and GHSR-1 distribution in the rat’s ocular tissue.

 
Methods:
 

Acute glaucoma was induced in male New Zealand white rabbits (2,0-3,0Kg; n=38) and male Wistar rats (200-300g; n= 17) through an intravitreous injection of 20% NaCl. Afterwards ghrelin was subconjunctivaly injected. In the rabbit, des-acyl ghrelin was also tested, as well as ghrelin’s effect in the presence of L-NAME or ketorolac. Immunolocalization of ghrelin and GHSR-1 was performed in male Wistar rats eye's (200-300g).

 
Results:
 

Ghrelin, but not des-acyl ghrelin, decreased the IOP in rabbit’s glaucomatous eyes (47.9±11.6%; figure 1). This effect was blunted in the presence of L-NAME and ketorolac. Ghrelin also decreased the IOP in rat’s glaucomatous eyes (41.4±12.2%). Ghrelin was detected in the inner part of ciliary processes, co-localized with lectin (figure 2), while GHSR-1 was detected in the ciliary muscle and choroid.

 
Conclusions:
 

Ghrelin promotes a decrease in the IOP in acute glaucomatous model eyes. This effect is mediated by GHSR-1a stimulation and prostaglandins or NO release. There appears to be a local system of production and action of Ghrelin and its related peptides.  

 

 
Keywords: ciliary body • ciliary processes • ciliary muscle 
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