March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Doxycycline'S Effect In Primary Pterygium Proteome Apoptosis
Author Affiliations & Notes
  • Victor M. Bautista, Sr.
    Microbiology and Ocular Proteomics, Inst de Ophthal Conde de Valenciana, Mexico, Mexico
  • Marilú Arredondo-Flores
    Microbiology and Ocular Proteomics,
    Hospital "Nuestra Señora de la Luz", Mexico, Mexico
  • Nadia Luz López-Espinosa
    Microbiology and Ocular Proteomics, Inst de Ophthal Conde de Valenciana, Mexico, Mexico
  • Teresa Valdéz-González
    Hospital "Nuestra Señora de la Luz", Mexico, Mexico
  • Herlinda Mejía-López
    Microbiology and Ocular Proteomics, Inst de Ophthal Conde de Valenciana, Mexico, Mexico
  • Footnotes
    Commercial Relationships  Victor M. Bautista, Sr., None; Marilú Arredondo-Flores, None; Nadia Luz López-Espinosa, None; Teresa Valdéz-González, None; Herlinda Mejía-López, None
  • Footnotes
    Support  Conde de Valenciana Foundation and CONACYT-126779
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5129. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Victor M. Bautista, Sr., Marilú Arredondo-Flores, Nadia Luz López-Espinosa, Teresa Valdéz-González, Herlinda Mejía-López; Doxycycline'S Effect In Primary Pterygium Proteome Apoptosis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5129.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Pterygium is a benign and fibrovascular lesion, originated from the bulbar conjunctiva. In pterygium has been described abnormal expression of apoptotic genes. We recently described that apoptosis is inhibited by the overexpression of peroxiredoxin 2 and anti-apoptotic proteins like Hsp-70, that block cytochrome c release and caspase activation, it results in a pro-caspase 3 accumulation. Some reports showed that doxycycline has pro-apoptotic effects activating caspase 3, 7 and 9, and inhibiting matrix metalloproteinase activity, and inducing cytochrome c release into cytosol. The aim of this work was to analyze in vivo the effect of doxycycline in pterygium proteome apoptosis

Methods: : Eigth patients with primary pterygia, previous informed consent, were treated with topic 2% doxycycline during two weeks and eigth no treated patients with primary pterygia and were analyzed by apoptosis protein array. Pterygia were rejected by surgery and were lysed. Two-hundred micrograms of proteins were incubated with the arrays and revealed with chemoluminiscence and spots density was quantified in G-BOX system. Fold change between pterygium treated and non-treated was obtained in order to establish relative protein expression. This study was approved by Scientific and Ethics Committees of Hospital "Nuestra Señora de la Luz" at Mexico City.

Results: : The analyzed results showed that in doxycycline treated pterygia 9 proteins were overexpressed, 2 proteins not had changes and 24 were down-expressed. Only catalase showed a fold change more than 2 times. Interestingly procaspase 3, clusterin, cytochrome c, Fas/TNFRSF6, HIF-1a, SMAC/DIABLO and caspase 3 were down expressed in comparison to previously report where we describe that these proteins were overexpressed in primary pterygium (ARVO 2011). The expression of bcl-2 and bcl-x decreased in doxycycline treated pterygia.

Conclusions: : We have described apoptosis inhibition in pterygium by overexpression of anti-apoptotic proteins and also reported the effect in vitro of doxycycline in pterygia cells, where doxycycline reduced cell viability and induced the production of TNF-alpha an others cytokines. In this work we describe the down expression of several apoptotic proteins by doxycycline's effect that suggest that apoptosis pathway in pterygium is recovered in contrast to apoptotic proteome described previously. Doxycycline could be an important part in the treatment of pterygium.

Clinical Trial: : Ethics Committee of Hospital "Nuestra Señora de la Luz", None

Keywords: pterygium • apoptosis/cell death • proteomics 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.