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Xiaoyan Chen; MicroRNA-206 Inhibits Uveal Melanoma Cell Proliferation and Migration by targeting c-Met. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5134.
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MicroRNAs (miRNAs) are 18-26 nucleotide non-coding RNAs which are expressed endogenously and repress protein translation through binding to target mRNAs. Evidence indicates that miRNAs are essential for tumor cell proliferation and migration. The function of miR-206 in uveal melanoma, however, remains unknown. In this study, we investigated the function of miR-206 in uveal melanoma cells.
Realtime RT-PCR was carried out to detect the expression of miR-206 in uveal melanoma cells and normal melanocytes. Transfection of miR-206 into uveal melanoma cells was performed by liperfectamine 2000. The proliferation of uveal melanoma cells was measured by MTS assay. Cell cycle analysis was performed by flow cytometry. Cell migration was examined by transwell migration assay. Western blot analysis was carried out to detect the expression of c-Met in uveal melanoma cells.
miR-206 was downregulated in uveal melanoma cells as compared with uveal melanocytes. Transfection of miR-206 into uveal melanoma cells significantly inhibited cell proliferation and migration. miR-206 induced cell cycle G1-arrest. c-Met expression was downregulated by miR-206 in uveal melanoma cells.
Our results demonstrated that miR-206 may act as a tumor suppressor in uveal melanoma cell proliferation and migration by targeting c-Met.
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