Purchase this article with an account.
Jungeun Lee, Jae Il Ahn, Jung-Ha Choi, Choun-Ki Joo; Arpe-19 Cells Survive During Tgf-β Induced EMT by Adopting Survivin. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5310.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Members of the transforming growth factor (TGF-β) superfamily are multifunctional cytokines that regulate cellular processes, including cell-cycle arrest, differentiation, morphogenesis, and apoptosis. TGF-β promotes extracellular matrix production and suppresses cell proliferation. Morphogenetic responses to TGF-β members include cell migration and epithelial-mesenchymal transitions (EMTs), which are critical during embryogenesis, development of fibrotic diseases, and advanced carcinoma spreading. The purpose of this study were to clarify how can survive human retinal pigment epithelial cells during TGF-β induced EMT.
Serum-starved ARPE-19 cells were incubated with vehicle alone or 10ng/ml TGF-β1 and we screened the expression of Survivin using RT-PCR and Western blot analysis in TGF-β1 treated cells. Furthermore, we confirmed this event using the Proteom profiler. Using siRNA targeting for Survivin, we show that these proteins are critical to TGF-β1 induced EMT.
RT-PCR, Western analysis and Proteom profiler was revealed that the expressions of survivin in ARPE-19 cells treated with TGF-β1. Using siRNA targeting for Survivin, we show that EMT marker is down regulated in TGF-β1 treated ARPE-19 cells lacking survivin compared to control cells treated with TGF-β1 only.
In conclusion, we showed that induction of EMT in human RPE cells led to up-regulation of Survivin expression, and inhibition of Survivin limited the development of EMT. We demonstrate that Survivin involves in the Transforming Growth Factor β1-mediated Epithelial-Mesenchymal Transition of retinal pigment epithelial cells.
This PDF is available to Subscribers Only