March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Arpe-19 Cells Survive During Tgf-β Induced EMT by Adopting Survivin
Author Affiliations & Notes
  • Jungeun Lee
    Catholic Institutes of Visual Science,
    The Catholic University of Korea, Seoul, Republic of Korea
  • Jae Il Ahn
    Catholic Institutes of Visual Science,
    The Catholic University of Korea, Seoul, Republic of Korea
  • Jung-Ha Choi
    Catholic Institutes of Visual Science,
    The Catholic University of Korea, Seoul, Republic of Korea
  • Choun-Ki Joo
    Catholic Institutes of Visual Science,
    Department of Ophthalmology & Visual Science, College of Medicine,
    The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Jungeun Lee, None; Jae Il Ahn, None; Jung-Ha Choi, None; Choun-Ki Joo, None
  • Footnotes
    Support  National Research Foundation of Korea(NRF) Grant 2011-0013562
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5310. doi:
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      Jungeun Lee, Jae Il Ahn, Jung-Ha Choi, Choun-Ki Joo; Arpe-19 Cells Survive During Tgf-β Induced EMT by Adopting Survivin. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5310.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Members of the transforming growth factor (TGF-β) superfamily are multifunctional cytokines that regulate cellular processes, including cell-cycle arrest, differentiation, morphogenesis, and apoptosis. TGF-β promotes extracellular matrix production and suppresses cell proliferation. Morphogenetic responses to TGF-β members include cell migration and epithelial-mesenchymal transitions (EMTs), which are critical during embryogenesis, development of fibrotic diseases, and advanced carcinoma spreading. The purpose of this study were to clarify how can survive human retinal pigment epithelial cells during TGF-β induced EMT.

Methods: : Serum-starved ARPE-19 cells were incubated with vehicle alone or 10ng/ml TGF-β1 and we screened the expression of Survivin using RT-PCR and Western blot analysis in TGF-β1 treated cells. Furthermore, we confirmed this event using the Proteom profiler. Using siRNA targeting for Survivin, we show that these proteins are critical to TGF-β1 induced EMT.

Results: : RT-PCR, Western analysis and Proteom profiler was revealed that the expressions of survivin in ARPE-19 cells treated with TGF-β1. Using siRNA targeting for Survivin, we show that EMT marker is down regulated in TGF-β1 treated ARPE-19 cells lacking survivin compared to control cells treated with TGF-β1 only.

Conclusions: : In conclusion, we showed that induction of EMT in human RPE cells led to up-regulation of Survivin expression, and inhibition of Survivin limited the development of EMT. We demonstrate that Survivin involves in the Transforming Growth Factor β1-mediated Epithelial-Mesenchymal Transition of retinal pigment epithelial cells.

Keywords: EMT (epithelial mesenchymal transition) • retinal pigment epithelium • proliferative vitreoretinopathy 
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