Purchase this article with an account.
Gabriela L. Ioshimoto, Balazs V. Nagy, Jan J. Kremers, Dora F. Ventura; Erg Changes In A Triple Transgenic Mouse Model For Alzheimer`s Disease. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5376.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To study the retinal function of the triple transgenic mouse model (3xTg-AD) for Alzheimer`s disease (AD) by comparing retinal electrophysiological responses in 3xTg-AD mice with those in the background control (b6;129-PS1). The responses were measured between 2 and 12 months of age.
ERGs were recorded from 44 3xTg-AD mice and from 23 background controls with a contact lens electrode on the cornea, a needle reference electrode on the head and a ground on the tail. Recordings were obtained for: 1) Maximum scotopic response (30cd.s/m2); 2) Light-adapted (30 cd/m2) flicker pulses (30 cd.s/m2) at 12, 18 and 30 Hz.
87% of control mice and 28% of the 3xTg-AD had very abnormal ERGs with a large b-wave implicit time (111,73 ± 22,56 ms) and no OPs. The others displayed ERGs with OPs and with b-wave implicit times within the range (45,31 ± 6,74 ms) expected from the literature. In the latter group, age dependent changes in the flash ERG were found for the a- and b-wave amplitudes. While the control group exhibited a mean decrease from 193,26 to 97,06 μV in the a-wave amplitude and a mean decrease from 452,4 to 230,71 μV in the b-wave amplitude between 6 and 12 months, 3xTg-AD group presented a low and constant response (a-wave= 143,4 ± 19,3 μV; b-wave= 303,5 ± 49,7 μV) between 6 and 12 months of age. Flicker amplitudes (1st harmonic after Fourier analysis) from the 3xTg-AD group were significantly reduced compared to controls at 6 months, but not at 12 months, both for the 12 Hz (p=0,0001) and for the 18 Hz (p=0,0001)stimuli.
Comparison of control and transgenic mice with ERGs with OPs and implicit times within the normal range, revealed a physiological impairment of the retina of AD mice. The a-wave amplitude decrease suggests that the effect involves loss or impairment of photoreceptor function. We conclude that AD may also affect the function of the retina.
This PDF is available to Subscribers Only