March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Retinopathy in a Cone-Rich Rodent Model of Spontaneous Type 2 Diabetes
Author Affiliations & Notes
  • Mandy Hong
    Ophthalmology,
    University of Alberta, Edmonton, Alberta, Canada
  • Michael B. Kreuzer
    Ophthalmology,
    University of Alberta, Edmonton, Alberta, Canada
  • Frederic P. Gaillard
    Ophthalmology,
    University of Alberta, Edmonton, Alberta, Canada
  • Sharee Kuny
    Ophthalmology,
    University of Alberta, Edmonton, Alberta, Canada
  • Kaiyuan Yang
    Nutrition,
    University of Alberta, Edmonton, Alberta, Canada
  • Miyoung Suh
    Nutrition, University of Manitoba, Winnipeg, Manitoba, Canada
  • Catherine Chan
    Nutrition,
    University of Alberta, Edmonton, Alberta, Canada
  • Yves Sauve
    Dept of Ophthalmology,
    University of Alberta, Edmonton, Alberta, Canada
  • Footnotes
    Commercial Relationships  Mandy Hong, None; Michael B. Kreuzer, None; Frederic P. Gaillard, None; Sharee Kuny, None; Kaiyuan Yang, None; Miyoung Suh, None; Catherine Chan, None; Yves Sauve, None
  • Footnotes
    Support  Alberta Innovates Health Solution (200700584); Canadian Diabetes Association (OG-1-09-2689-CC)
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5405. doi:
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    • Get Citation

      Mandy Hong, Michael B. Kreuzer, Frederic P. Gaillard, Sharee Kuny, Kaiyuan Yang, Miyoung Suh, Catherine Chan, Yves Sauve; Retinopathy in a Cone-Rich Rodent Model of Spontaneous Type 2 Diabetes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5405.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To characterize the impact of type 2 diabetes on retina anatomy and function in a novel rodent model of cone-based vision.

Methods: : Nile grass rats (Arvicanthis niloticus) were fed a standard rodent diet, and phenotyped for type 2 diabetes (urine and plasma glucose, islet morphology). Retinas were studied anatomically and functionally (full field electroretinogram, ERG) from 1 to 18 months.

Results: : The severity of retina anatomical and functional defects was correlated with age, representing the progression of type 2 diabetes. Anatomical studies at 15-18 months revealed: retinal vascular abnormalities (acellular capillaries, pericyte dropout, tortuous vessels), retinal gliosis and central retina edema. Retinal dysfunction first consisted of reduced cone-driven oscillatory potential (OP) amplitudes (a well-established precocious marker of diabetic retinopathy) by 3 months of age. Inner retinal dysfunction (OP and b-wave amplitude reductions) further progressed with age in Nile rats, as inferred by reduced ERG b/a wave amplitude ratios. Outer retina defects (evidenced by a-wave amplitude reductions) affected rods by 12 months and then cones by 15-18 months.

Conclusions: : Our data support the pertinence of the Nile grass rat as a progressive, cone-rich model of diabetic retinopathy with central retina edema resulting from spontaneous type 2 diabetes.

Keywords: electroretinography: non-clinical • diabetes • anatomy 
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