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Mandy Hong, Michael B. Kreuzer, Frederic P. Gaillard, Sharee Kuny, Kaiyuan Yang, Miyoung Suh, Catherine Chan, Yves Sauve; Retinopathy in a Cone-Rich Rodent Model of Spontaneous Type 2 Diabetes. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5405.
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© ARVO (1962-2015); The Authors (2016-present)
To characterize the impact of type 2 diabetes on retina anatomy and function in a novel rodent model of cone-based vision.
Nile grass rats (Arvicanthis niloticus) were fed a standard rodent diet, and phenotyped for type 2 diabetes (urine and plasma glucose, islet morphology). Retinas were studied anatomically and functionally (full field electroretinogram, ERG) from 1 to 18 months.
The severity of retina anatomical and functional defects was correlated with age, representing the progression of type 2 diabetes. Anatomical studies at 15-18 months revealed: retinal vascular abnormalities (acellular capillaries, pericyte dropout, tortuous vessels), retinal gliosis and central retina edema. Retinal dysfunction first consisted of reduced cone-driven oscillatory potential (OP) amplitudes (a well-established precocious marker of diabetic retinopathy) by 3 months of age. Inner retinal dysfunction (OP and b-wave amplitude reductions) further progressed with age in Nile rats, as inferred by reduced ERG b/a wave amplitude ratios. Outer retina defects (evidenced by a-wave amplitude reductions) affected rods by 12 months and then cones by 15-18 months.
Our data support the pertinence of the Nile grass rat as a progressive, cone-rich model of diabetic retinopathy with central retina edema resulting from spontaneous type 2 diabetes.
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