March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Prevention Of Endotoxin-induced Uveitis By Anti-diabetic Drug Metformin In Rats
Author Affiliations & Notes
  • Kota V. Ramana
    Biochemistry and Molecular Biology,
    Univ Texas Medical Branch, Galveston, Texas
  • Nilesh M. Kalariya
    Ophthalmology,
    Univ Texas Medical Branch, Galveston, Texas
  • Mohammad Shoeb
    Biochemistry and Molecular Biology,
    Univ Texas Medical Branch, Galveston, Texas
  • Naseem H. Ansari
    Biochemistry and Molecular Biology,
    Univ Texas Medical Branch, Galveston, Texas
  • Satish K. Srivastava
    Biochemistry and Molecular Biology,
    Univ Texas Medical Branch, Galveston, Texas
  • Footnotes
    Commercial Relationships  Kota V. Ramana, None; Nilesh M. Kalariya, None; Mohammad Shoeb, None; Naseem H. Ansari, None; Satish K. Srivastava, None
  • Footnotes
    Support  EY015891
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5486. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kota V. Ramana, Nilesh M. Kalariya, Mohammad Shoeb, Naseem H. Ansari, Satish K. Srivastava; Prevention Of Endotoxin-induced Uveitis By Anti-diabetic Drug Metformin In Rats. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5486.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Metformin is a commonly used anti-diabetic drug in patients because of its well established efficacy and negligible side effects. Its efficacy in preventing ocular inflammatory diseases is not known. Hence, we investigated anti-ocular inflammatory effects of Metformin in endotoxin-induced uveitis (EIU) in rats.

Methods: : EIU was induced by subcutaneous injection of lipopolysaccharide (LPS) (150 ug) in Lewis rats treated with or without metformin. The number of infiltrating cells and levels of proteins were determined in the aqueous humor (AqH). The MILLIPLEX MAG Rat cytokine/chemokine magnetic bead array was used to determine the levels of various cytokines and chemokines in AqH. Human primary non-pigment ciliary epithelial cells (HNPECs) were used to determine anti-inflammatory efficacy in vitro.

Results: : Compared to controls, the EIU rat eyes AqH had significantly higher number of infiltrating cells, total protein, and various cytokines and chemokines (TNF-α, MCP-1, IL-1β, MIP-1α, IL-6, Leptin, IL-18, and GRO/KC) and metformin significantly prevented these alterations. Metformin also prevented the expression of Cox-2 and activation of NF- B, and increased the activation of AMPK in the ciliary bodies and retinal tissues. Moreover, metformin also prevented the expression of Cox-2, iNOS, and phosphorylation of NF- B in HNPECs and decreased the levels of Nitrate/Nitrite and PGE2 in cell culture media.

Conclusions: : Our results for the first time demonstrate a novel role of anti-diabetic drug, metformin, in suppressing uveitis in rats and suggest that it could be developed as a potential agent to prevent uveitis complications.

Keywords: uveitis-clinical/animal model • inflammation • oxidation/oxidative or free radical damage 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×