March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
A Novel Method To Transfect Retinal Pigment Epithelial Cells Without Detaching The Retina
Author Affiliations & Notes
  • Francine F. Behar-Cohen
    Ophthalmology, Hotel Dieu de Paris, Universite Paris Descartes, Paris, France
    Physiopathology ocular diseases,
    Inserm UMRS872, Paris, France
  • Elodie Touchard
    Physiopathologie of ocular dseases,
    Inserm UMRS872, Paris, France
  • Berdugo Marianne
    Physiopathology of ocular diseases,
    Inserm UMRS872, Paris, France
  • Michèle Savoldelli
    Ophthalmology, Hotel Dieu de Paris, Universite Paris Descartes, Paris, France
  • Marie-Christine Naud
    Physiopathology ocular diseases,
    Inserm UMRS872, Paris, France
  • Jean-Claude Jeanny
    Physiopathology ocular diseases,
    Inserm UMRS872, Paris, France
  • Footnotes
    Commercial Relationships  Francine F. Behar-Cohen, inventor (P); Elodie Touchard, Inventor (P); Berdugo Marianne, None; Michèle Savoldelli, None; Marie-Christine Naud, None; Jean-Claude Jeanny, None
  • Footnotes
    Support  Fondation Recherche Médicale
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5580. doi:
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      Francine F. Behar-Cohen, Elodie Touchard, Berdugo Marianne, Michèle Savoldelli, Marie-Christine Naud, Jean-Claude Jeanny; A Novel Method To Transfect Retinal Pigment Epithelial Cells Without Detaching The Retina. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5580.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Sub retinal route of viral vectors is the validated method to transfect retinal cells at the site of retinal detachment. A safer method would benefit to macular targeting. We have evaluated a novel method that combines the administration of plasmid DNA in the suprachoroidal space of rat eyes with scleral electroporation procedure.

Methods: : Optimal injection, current electroporation parameters and electrode geometry were evaluated using a plasmid encoding Lac-Z. Safety of the procedure was monitored using fluorescein angiography, electroretinography and histology.

Results: : Suprachoroidal injection induces a large area of diffusion. Scleral electroporation using external electrodes efficiently transfects not only choroidal cells but also retinal pigment epithelial cells and photoreceptors using a CMV promoter. No histologic or functional resulted from this minimally invasive procedure

Conclusions: : We describe here a novel minimally invasive, non-viral gene therapy method for the transfection of RPE cells without any retinal detachment. Further analysis will be performed to optimize cell specific targeting and long-term expression.

Keywords: gene transfer/gene therapy • retinal pigment epithelium • retina 
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