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Elvire Vaucher, Mylène Pouliot, Tamara M. Boutin, Olivier Fontaine, Rejean Couture; Vascular Alteration And Lipids Accumulation In The Retina And Choroid Of Non-insulin-dependent Diabetic Goto-Kakizaki Rats. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5773. doi: https://doi.org/.
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To examine whether the non-insulin-dependent diabetic Goto-Kakizaki (GK) rats develop retinal vascular alterations. Microvascular bed morphological alterations as well as lipid deposits in the retina and choroid were particularly evaluated. Endothelial function integrity was measured by assessing retinal vascular permeability.
30- and 36-week male GK rats, an animal model of spontaneous type 2 diabetes without obesity and age-matched Wistar rats were used. The retinal vascular bed was examined using ADPase staining. The level of lipid accumulation was graded using triglycerides staining with Oil red O. The retinal vascular permeability was assessed using Evans blue dye extravasation technique. Student’s t-test was used to determine significant difference between control and GK rats.
Staining of the retinal sections with Oil Red O revealed abundant lipid deposits between the retina and choroid of GK rats but not in control animals. This was accompanied by greater tortuosity of the retinal microvessels, an index of endothelial dysfunction. However, retinal vessels density (% of vessels/surface area: 23.4 ± 0.5 vs. 22.2 ± 0.5, P > 0.05) and vascular permeability (Evans blue, µg/g of fresh tissue: 22.8 ± 4.3 vs. 22.0 ±2.3, P > 0.05) were not changed in 30- and 36-GK rats compared to controls.
This results show that lipids accumulation occurs in the retina and choroid of spontaneous type 2 diabetic retina which might be linked to vascular alterations. Endothelial dysfunction in GK rats was detected by presence of vessel tortuosity but was not associated with a change in vascular permeability and vessel density.
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