Abstract
Purpose: :
To investigate the efficacy and feasibility of CXCR4 inhibitor(AMD3100)combined with anti-VEGF antibody in laser-induced choroidal neovascularization in Brown-Norway (BN) rats.
Methods: :
Choroidal neovascularization was induced in 48 BN rats were randomly divided into 4 groups (n=12 eyes). Right after laser photocoagulation, each group respectively received an intravitreal injection of 5μl of anti-rat VEGF antibody (AF564), AMD3100, both AF564 and AMD3100, PBS as control.
Results: :
14 days after photocoagulation, the leakage of fluorescein in the treated groups (including AF564 group, AMD3100 group, combined group) weakened than the control group (P<0.001), the combined group showed less fluorescein leakage than that in the AF564 group or the AMD3100 group (P<0.05). The FITC-fluorescent-tagged CNV area were significantly decreased in all treated groups, especially the combined group (P<0.001). The CNV area is (8204±122)µm2, (9332±211)µm2, (6533±101)µm2 and (13644±255)µm2 respectively in the AF564 group, AMD3100 group, combined group and the control group.
Conclusions: :
Combinatory inhibition of CXCR4 inhibitor and anti-VEGF antibody successfully inhibited laser-induced CNV, suggesting that the combinatory treatment may be beneficial in the clinical treatment of CNV.
Keywords: choroid: neovascularization • vascular endothelial growth factor • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound)