March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Functional Recovery After Experimental RPE Debridement, mfERG Studies in a Porcine Model
Author Affiliations & Notes
  • Jens F. Kiilgaard
    Dept of Ophthalmology, Rigshospitalet, Copenhagen, Denmark
    Dept. of Ophthalmology, Glostrup Copenhagen Univ. Hospital, Glostrup, Denmark
  • Nina Sørensen
    Dept. of Ophthalmology, Glostrup Copenhagen Univ. Hospital, Glostrup, Denmark
  • Maria V. Kyhn
    Dept. of Ophthalmology, Glostrup Copenhagen Univ. Hospital, Glostrup, Denmark
  • Natan Lassota
    Eye Pathology Inst, Copenhangen University, Copenhagen, Denmark
  • Jan U. Prause
    Eye Pathology Inst, Copenhangen University, Copenhagen, Denmark
  • Morten D. de la Cour
    Dept. of Ophthalmology, Glostrup Copenhagen Univ. Hospital, Glostrup, Denmark
  • Footnotes
    Commercial Relationships  Jens F. Kiilgaard, None; Nina Sørensen, None; Maria V. Kyhn, None; Natan Lassota, None; Jan U. Prause, None; Morten D. de la Cour, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5836. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jens F. Kiilgaard, Nina Sørensen, Maria V. Kyhn, Natan Lassota, Jan U. Prause, Morten D. de la Cour; Functional Recovery After Experimental RPE Debridement, mfERG Studies in a Porcine Model. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5836.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract 
 
Purpose:
 

The goal of this study is to ascertain the functional impact of RPE debridement. We have investigated the time-dependent correlation between histopathological appearance and mfERG results, after RPE removal in an animal model.

 
Methods:
 

All experiment was carried out in accordance with the ARVO Statement on the use of animals in ophthalmic and vision research, and approved by the Danish Animal Experiment Inspectorate. 10 female domestic pigs were anaesthetised, endotracheally intubated and artificially ventilated. Access to the subretinal space was achieved by a central and posterior three-port vitrectomy followed by fluid injection into the subretinal space causing a retinal detachment bleb. Hereafter was the RPE debrided gently by brushing Bruch's membrane with a soft-tip silicone catheter. The impact of the RPE debridement was evaluated immediately after surgery and after two and six weeks by; 1) MfERG (VERIS Science 5.0.1) (first examination was performed at baseline, none immediately after surgery), 2) fundus photographs, 3) fluorescein angiograms and 4) histopathological examination.

 
Results:
 

The mean P1 amplitude, calculated with a one-way ANOVA, was at baseline 1,995 mV/dg2, it decreased to 1,278 mV/dg2 one week after surgery and 1,388 mV/dg2 two weeks after surgery. Six weeks after surgery it had increased to 2,169 mV/dg2 .Multivariate analysis for the three endpoints showed no significant difference between one and two weeks after surgery. The mfERG P1 amplitude one and two weeks after surgery is significantly decreased compared to baseline and six weeks after surgery. The mean P1 amplitude at six weeks is not significantly different from baseline.

 
Conclusions:
 

This is the first study to show that regeneration of RPE, in an animal model of RPE debridement, restores the retinal function. In our porcine model, when RPE migrates to the debrided area, the mfERG normalizes. Thus, the function of the photoreceptors in the healed area is restored.  

 
Keywords: retinal pigment epithelium • electroretinography: clinical • regeneration 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×