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Felix Y. Chau, Olachi J. Mezu-Ndubuisi, Narsa M. Reddy, Justin Wanek, Pang-yu Teng, Norman P. Blair, Sekhar P. Reddy, Mahnaz Shahidi; Spectral Domain Optical Coherence Tomography (SDOCT) Findings in a Mouse Model of Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5885.
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© ARVO (1962-2015); The Authors (2016-present)
Retinopathy of prematurity (ROP) is a multifactorial and potentially blinding eye disease. Retinal vascular obliteration has been demonstrated ex vivo in an oxygen induced retinopathy (OIR) mouse model of ROP. The purpose of this study is to report abnormalities in retinal thickness and vascular structure in vivo due to OIR by fluorescein angiography (FA) and spectral domain optical coherence tomography (SDOCT).
Three wildtype (C57BL6/J) newborn mice pups were exposed to 5 days of 75% oxygen from P7 to P12 and subsequently returned to room air (OIR), while 4 mice were continually kept at room air (control). At P17, FA and SDOCT were performed to visualize retinal vasculature and measure retinal thickness. Retinal thickness maps were generated from B-scan rasters acquired at peripheral retinal areas, approximately 3 disk diameters from the optic disk center. Retinal thickness measurements obtained in OIR and control mice were compared.
In all control mice, retinal thickness was uniform on peripheral SDOCT B-scans and thickness maps. In 2 of 3 OIR mice, peripheral SDOCT B-scans and thickness maps displayed both regions with normal and reduced retinal thickness. In regions with retinal thinning, the inner retina showed reduced thickness, while the outer retinal layers were not affected. Mean total retinal thickness (N = 5) in OIR mice in areas of thinning was on average 82% of the mean thickness (N = 11) in control mice. In both control and OIR mice, B-scans displayed an increase in reflectivity from the vitreoretinal interface at the optic disks and from retinal vessels. Near retinal blood vessels, retinal thicknesses measured in OIR and control mice were similar.
Retinal thickness and vascular features were successfully characterized in an OIR mouse model of ROP using SDOCT. Retinal thinning observed in OIR mice likely corresponds to regions of capillary nonperfusion and inner retinal atrophy due to ischemia. In vivo monitoring of retinal thickness and vascular features in OIR may be useful for evaluating therapies for human ROP.
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