March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Decreased IGF1 Expression Associated with Avascular Retina in Model of Retinopathy of Prematurity
Author Affiliations & Notes
  • Yanchao Jiang
    Ophthalmology, John Moran Eye Center, The University of Utah, Salt Lake City, Utah
  • Ben Numpang
    Division of Neonatology, The University of Utah, Salt Lake City, Utah
  • Baifeng Yu
    Division of Neonatology, The University of Utah, Salt Lake City, Utah
  • Haibo Wang
    Ophthalmology, John Moran Eye Center, The University of Utah, Salt Lake City, Utah
  • George W Smith
    Ophthalmology, John Moran Eye Center, The University of Utah, Salt Lake City, Utah
  • Manabu McCloskey
    Ophthalmology, John Moran Eye Center, The University of Utah, Salt Lake City, Utah
  • Shrena Patel
    Division of Neonatology, The University of Utah, Salt Lake City, Utah
  • Robert DiGeronimo
    Division of Neonatology, The University of Utah, Salt Lake City, Utah
  • ME Hartnett
    Ophthalmology, John Moran Eye Center, The University of Utah, Salt Lake City, Utah
  • Robert Lane
    Division of Neonatology, The University of Utah, Salt Lake City, Utah
  • Footnotes
    Commercial Relationships  Yanchao Jiang, None; Ben Numpang, None; Baifeng Yu, None; Haibo Wang, None; George W Smith, None; Manabu McCloskey, None; Shrena Patel, None; Robert DiGeronimo, None; ME Hartnett, None; Robert Lane, None
  • Footnotes
    Support  RO1EY015130 RO1EY017011 and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5888. doi:
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      Yanchao Jiang, Ben Numpang, Baifeng Yu, Haibo Wang, George W Smith, Manabu McCloskey, Shrena Patel, Robert DiGeronimo, ME Hartnett, Robert Lane; Decreased IGF1 Expression Associated with Avascular Retina in Model of Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5888.

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Abstract

Purpose: : Low serum insulin like growth factor 1 (IGF1) has been reported to be associated with avascular retina and severe retinopathy of prematurity (ROP) in human preterm infants. We used a rat model of ROP to address the hypothesis that components in IGF1 signaling pathway would be reduced in the retina of rats in the ROP model when avascular retina persisted compared to rats raised in room air (RA).

Methods: : Within 4 hours of birth, newborn rat pups with dams were placed into an oxygen environment that cycled between 50% and 10% every 24 hours for 14 days. Pups raised at RA were used as control. At postnatal day (p) 14, pups were euthanized, and retinas were dissected from eyes and analyzed by real-time polymerase chain reaction for IGF1 variants, IGF1 receptor (IGF1R) mRNAs and by western blot for IGF1 and IGF1R protein.

Results: : At p14, retinas from the ROP model had significantly decreased expression of IGF1A, IGF1B and IGF1R mRNAs compared to retinas from RA (p=0.0015 for IGF1A vs. RA, p=0.0142 for IGF1B vs. RA, p=0.0004 for IGF1R vs. RA, respectively). IGF1 protein was decreased significantly in retinas taken from rats exposed to the ROP model compared to RA (p=0.0019), whereas IGF1R was increased (p=0.014) in retinas from the ROP model compared to RA.

Conclusions: : Retinal IGF1 expression is reduced in both mRNA and protein levels in ROP model compared to RA at p14. IGF1R mRNA is reduced whereas its protein is increased compared to RA. These data show an association between reduced IGF1 signaling components and avascular retina in a relevant animal model of ROP.

Keywords: retinopathy of prematurity • retinal development • hypoxia 
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