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Yanchao Jiang, Ben Numpang, Baifeng Yu, Haibo Wang, George W Smith, Manabu McCloskey, Shrena Patel, Robert DiGeronimo, ME Hartnett, Robert Lane; Decreased IGF1 Expression Associated with Avascular Retina in Model of Retinopathy of Prematurity. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5888.
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Low serum insulin like growth factor 1 (IGF1) has been reported to be associated with avascular retina and severe retinopathy of prematurity (ROP) in human preterm infants. We used a rat model of ROP to address the hypothesis that components in IGF1 signaling pathway would be reduced in the retina of rats in the ROP model when avascular retina persisted compared to rats raised in room air (RA).
Within 4 hours of birth, newborn rat pups with dams were placed into an oxygen environment that cycled between 50% and 10% every 24 hours for 14 days. Pups raised at RA were used as control. At postnatal day (p) 14, pups were euthanized, and retinas were dissected from eyes and analyzed by real-time polymerase chain reaction for IGF1 variants, IGF1 receptor (IGF1R) mRNAs and by western blot for IGF1 and IGF1R protein.
At p14, retinas from the ROP model had significantly decreased expression of IGF1A, IGF1B and IGF1R mRNAs compared to retinas from RA (p=0.0015 for IGF1A vs. RA, p=0.0142 for IGF1B vs. RA, p=0.0004 for IGF1R vs. RA, respectively). IGF1 protein was decreased significantly in retinas taken from rats exposed to the ROP model compared to RA (p=0.0019), whereas IGF1R was increased (p=0.014) in retinas from the ROP model compared to RA.
Retinal IGF1 expression is reduced in both mRNA and protein levels in ROP model compared to RA at p14. IGF1R mRNA is reduced whereas its protein is increased compared to RA. These data show an association between reduced IGF1 signaling components and avascular retina in a relevant animal model of ROP.
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