March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Gene Expression and Immunogenicity of induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • Hiroyuki Kamao
    Laboratory for Retinal Regeneration, RIKEN Ctr for Dvlpmntl Biology, Kobe, Japan
    Ophthalmology, Kawasaki medical school, Okayama, Japan
  • Michiko Mandai
    Laboratory for Retinal Regeneration, RIKEN Ctr for Dvlpmntl Biology, Kobe, Japan
  • Akiko Suga
    Laboratory for Retinal Regeneration, RIKEN Ctr for Dvlpmntl Biology, Kobe, Japan
  • Junichi Kiryu
    Ophthalmology, Kawasaki medical school, Okayama, Japan
  • Masayo Takahashi
    Laboratory for Retinal Regeneration, RIKEN Ctr for Dvlpmntl Biology, Kobe, Japan
  • Footnotes
    Commercial Relationships  Hiroyuki Kamao, None; Michiko Mandai, None; Akiko Suga, None; Junichi Kiryu, None; Masayo Takahashi, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 5893. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hiroyuki Kamao, Michiko Mandai, Akiko Suga, Junichi Kiryu, Masayo Takahashi; Gene Expression and Immunogenicity of induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):5893.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To evaluate the gene expression and immunogenicity of human and monkey induced pluripotent stem cell-derived retinal pigment epithelial cells (iPSC-RPEs).

Methods: : The human and monkey iPSCs were generated and iPSC-RPEs were induced with modified SFEB method. We evaluated the expression of 154 genes that had been reported as RPE signature genes in the 10 lines of human iPSC-RPEs by microarray analysis. The expression levels of MHC-I and II on the surface of the human iPSCs-RPEs with or without interferon-gamma (IFN-γ) were also studied by flow cytometry. The monkey iPSC-RPE sheets with basement membranes were transplanted into the subretinal space as allograft (3 monkeys) and as autograft (1 monkey). Results were evaluated with fundus photographs, fluorescein angiography (FA) and optical coherence tomography (OCT) at 1 month and 1 year after transplantation.

Results: : Compared to primary human fetal RPE cells or ARPE19 cells as control, all the hiPSC-RPE lines unanimously demonstrated a similar expression pattern of RPE signature genes. The hiPSC-RPEs expressed similar levels of MHC-I with and without IFN-γ stimulation. On the other hand, the addition of IFN-γ up-regulated MHC-II expression in the hiPSC-RPEs. All the allografts showed signs of rejection such as fibrous scar tissues, retinal edema, fluorescein leakage in FA and hyperreflective material under the retina in OCT, whereas the autograft showed no such evidence of rejection until1 year after surgery.

Conclusions: : We showed that autologous hiPSC-RPEs were suitable for clinical application in terms of gene expression and immunoreaction. We should confirm these results by increasing the number of monkey iPSC-RPEs transplantations.

Keywords: retinal pigment epithelium • age-related macular degeneration • transplantation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×