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Toru Matsunaga, Yasuo Watanabe, Takao Sato, Toshinari Funaki, Akira Matsuda, Nobuyuki Ebihara, Akira Murakami; Investigation for the Possibility of Using Polymer Hydrogels as a Device for Cultivation and Transplantation of Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6039.
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© ARVO (1962-2015); The Authors (2016-present)
Amnion of placental tissue is widely used as a device for the transplantation of corneal epithelial cells. However, including other devices, this device has problems with safety and supply stability. Thus, development of novel devices is expected. So, we use contact lenses which are composed of hydrogels with the phosphate group side chains (P-CL) as a device, and examined cultivation and transplantation of corneal epithelial cells.
SV40 immortalized human corneal epithelial cells (HCE-T) was used, and cultured HCE-T cells on P-CL and various commercial CLs in vitro. Furthermore, we collected pieces of the tissue from the limbus corneae of GFP transgenic rabbit, and cultured the monolayer of stem cells on CLs. After that, keep the condition of monolayer culture on CLs, these CLs were worn on Japanese white rabbit eyes whose corneal epithelial cells were stripped, and evaluated the histological specimen with fluorescence microscope.
The results of cultivation of HCE-T on CL in vitro differed depending on lens materials. Regarding P-CL, the growth of cells collected from the limbus corneae of GFP transgenic rabbits were good, and expression of undifferentiated cell marker (p63) by immunostaining was also recognized. Furthermore, regarding Japanese white rabbit eyes which wore the monolayer cultured CL, the migration of cells was confirmed.
As a result, CL can be used as a device for cultivation of corneal epithelial cells and transplanted for corneal epithelial. Therefore, P-CL was suggested to be useful as a device for transplantation of corneal epithelial cell.
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