March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Hyaluronan Release from Contact Lenses in vitro and in vivo
Author Affiliations & Notes
  • Krista M. Fridman
    Bausch + Lomb, Rochester, New York
  • Catherine A. Scheuer
    Bausch + Lomb, Rochester, New York
  • Stephanie Su
    Bausch + Lomb, Rochester, New York
  • Lening Zhang
    Bausch + Lomb, Rochester, New York
  • Susan E. Burke
    Bausch + Lomb, Rochester, New York
  • Footnotes
    Commercial Relationships  Krista M. Fridman, Bausch + Lomb (E); Catherine A. Scheuer, Bausch + Lomb (E); Stephanie Su, Bausch + Lomb (E); Lening Zhang, Bausch + Lomb (E); Susan E. Burke, Bausch + Lomb (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6099. doi:
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    • Get Citation

      Krista M. Fridman, Catherine A. Scheuer, Stephanie Su, Lening Zhang, Susan E. Burke; Hyaluronan Release from Contact Lenses in vitro and in vivo. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6099.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Hyaluronan (HA), a natural ocular lubricant, provides hydration and contributes to tear film stabilization. This study evaluates in vitro contact lens adsorption and release of HA after soaking in multi-purpose solution containing HA. Further, this study examined the concentration of HA in tear film of lens wearers after use of MPS containing HA.

Methods: : In vitro adsorption and release of HA from senofilcon A lenses (Acuvue Oasys, Vistakon) were evaluated with fluorescence spectroscopy. Lenses were soaked in MPS (Biotrue, Bausch+Lomb) prepared with fluorescent-tagged HA. Release profiles were monitored over time with saline rinse of soaked lenses at the rate of tear film secretions. In vivo release of HA from senofilcon A lenses soaked in MPS containing HA (Biotrue, Bausch+Lomb) was evaluated with ELISA. Twenty five subjects matched for age and sex (12 habitual lens wearers and 13 non-lens wearers) participated in this randomized, single-masked, contralateral study. Tears were collected on Schirmer strips at baseline and after 2h of wearing MPS soaked lenses or control lenses rinsed with Sensitive Eyes Saline. HA concentrations were measured and adjusted for sample volumes. A repeated measurement ANOVA model was used to evaluate differences in HA concentrations.

Results: : In vitro measurements showed adsorbed HA (19.72+/-2.03 µg HA/lens) was released over time from senofilcon A. In vivo HA tear concentrations obtained were in range with those previously reported (undetectable - 3018ng HA/ml tears). No statistical differences were noted in this study population between lens wearers and non-lens wearers, between baselines of treatment groups, or between saline treatment group and baseline. An increase in HA concentration was observed in tears collected from eyes that had worn lenses soaked in MPS containing HA compared to baseline (p=0.009), and compared to those with saline rinsed control contact lenses (p=0.033).

Conclusions: : This study showed that senofilcon A lenses adsorbed HA when soaked in MPS containing HA and that HA release was detected for 20h when rinsed in vitro at the rate of tear film secretions. This study also showed that after wearing senofilcon A lenses soaked in an HA containing MPS, the HA concentration in tear film may be increased. The duration of in vivo HA increase was not evaluated.

Clinical Trial: : http://www.clinicaltrials.gov 01317030

Keywords: contact lens • clinical laboratory testing • aqueous 
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