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Barbara Teuchner, Eduard Schmid, Markus Nagl, Nikolaos Bechrakis; N-chlootaurine,n-monochloro-deimethyltaurine And N,n-dichloro-dimethyltaurine Are Safe And Effective Bacteriacidal Agents In Cornea Models. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6200.
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N-chlorotaurine (NCT), NVC-422 (N,N-dichloro-dimethyltaurine) and NVC-612 (N-monochloro-dimethyltaurine) are potential anti-infective agents useful for the treatment of conjunctivitis and keratitis. The aim of this study was to show that these compounds are safe in an EpiOcular model and effective in corneas infected ex vivo with P. aeruginosa and S. aureus.
Corneal discs were punched with a Trepan from eyes from Tyrolean farm pigs and scarified to produce artificial erosions. Discs were incubated with bacteria and subsequently washed with saline. The different test compounds were applied to the corneas in phosphate buffer at pH 7.1. Discs were homogenized followed by quantitative bacterial cultures or subjected to histological preparation. Ocular irritancy was tested using the EpiOcular™ tissue system (Mattek Corporation).
In histological sections, bacteria attached to the surface, and accumulations of bacteria in the upper third of the stroma could be seen. All test compounds, 55 mM NCT, 55 mM NVC-422, 55 mM NVC-612, 11 mM NCT+ 37 mM NH4Cl, or 5.5 mM NCT+ 18.5 mM NH4Cl, reduced the bacterial counts by approximately 5 log10 after 60 min (P. aeruginosa) and 120 min (S. aureus) incubation. Significant cidal activity occurred already after 5 min incubation and increased over time. While surface bacteria were killed by using 70% ethanol for 0.5 min giving a 0.5-1 log10 kill, additional treatment with the test compounds resulted in kill to the limit of detection suggesting that the test compounds killed tissue resident bacteria. Using an EpiOcular™ tissue model developed for in vitro irritancy testing (Draize score), we further show that NCT, NVC-422 and NVC-612 have no or very low irritancy potential to corneal tissue in this study.
The results demonstrate that NCT, NVC-422, and NVC-612 have the ability to kill P. aeruginosa and S. aureus and are non-irritating in cornea.
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