March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Biocidal Activity of a Novel One-Step Hydrogen Peroxide Disinfecting Solution and Comparator Peroxide Disinfecting Solutions against Ocular Pathogens Including Multidrug-Resistant Bacteria
Author Affiliations & Notes
  • Deborah McGrath
    Microbiology, Bausch & Lomb, Inc., Rochester, New York
  • Damien W. Cordero
    Microbiology, Bausch & Lomb, Inc., Rochester, New York
  • Patricia A. Walsh
    Microbiology, Bausch & Lomb, Inc., Rochester, New York
  • Timothy W. Morris
    Microbiology, Bausch & Lomb, Inc., Rochester, New York
  • Footnotes
    Commercial Relationships  Deborah McGrath, Bausch & Lomb, Inc. (E); Damien W. Cordero, Bausch & Lomb, Inc. (E); Patricia A. Walsh, Bausch & Lomb, Inc. (E); Timothy W. Morris, Bausch & Lomb, Inc. (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6212. doi:
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      Deborah McGrath, Damien W. Cordero, Patricia A. Walsh, Timothy W. Morris; Biocidal Activity of a Novel One-Step Hydrogen Peroxide Disinfecting Solution and Comparator Peroxide Disinfecting Solutions against Ocular Pathogens Including Multidrug-Resistant Bacteria. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6212.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Compendial biocidal efficacy testing of contact lens disinfection solutions is currently limited to 5 ISO/FDA specified microbial strains. The current study was conducted to more broadly evaluate the biocidal activity of one-step hydrogen peroxide disinfection systems against a set of 15 non-compendial ocular pathogen strains, including methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistantStaphylococcus epidermidis (MRSE) and quinolone-resistant Pseudomonas aeruginosa (QRPA).

Methods: : Stand-alone biocidal efficacy test methods were based upon the ISO 14729 test standard for disinfecting products modified to permit testing of both hospital-acquired (HA) and community-acquired (CA) MRSA isolates as well as QRPA, MRSE, Stenotrophomonas maltophilia, Serratia marcescens, Achromobacter xylosidans, Escherichia coli, Listeria monocytogenes, Klebsiella pneumoniae, Ralstonia pickettii, and Streptococcus pneumoniae. Challenge inocula were prepared in 10% organic soil, diluted to ~ 5 x 105 CFU/mL into peroxide neutralizing lens cases containing 10 mL fresh solutions. A novel one-step hydrogen peroxide disinfecting solution along with Clear Care and Oxysept comparators were all tested in recommended companion lens cases for each solution. Inoculated lens cases were sampled at 4 and 6 hr, neutralized with catalase to remove any residual peroxide, and plated to enumerate surviving bacteria and calculate log reduction values.

Results: : All three peroxide solutions showed little or no recovery of surviving cells for all 15 bacterial strains at either 4 or 6 hr time points, with log reduction values ranging from > 3.6 to > 5.2.

Conclusions: : The results indicate that all three one-step peroxide disinfection systems show strong, broad spectrum in vitro activity against this extensive range of pathogenic bacteria, including MRSA, MRSE, and QRPA isolates that exhibit resistance to a variety of therapeutic antibacterial drugs.

Keywords: keratitis • contact lens • bacterial disease 
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