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Marina Nikolic, Simon Kilvington, Anthony Lam, Nancy Brady; Resistance And Growth Of Fusarium spp. In Contact Lens Disinfectant Solutions. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6213.
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Fusarium solani and F. oxysporum can cause fungal keratitis in contact lens wearers and an outbreak of infection in 2005-2006 was attributed to use of a single multipurpose disinfectant solution (MPS). ISO14729 requires a 1 Log kill of F. solani (ATCC 36031) within the manufacturer’s recommended disinfection time to meet the stand-alone biocidal criteria. Here, the efficacy of contact lens care solutions against clinical isolates of Fusarium spp. was investigated.
Care solutions and their biocidal agents were: MPS-1 (PQ-1 with alexidine), MPS-2 (PQ-1 with PHMB), MPS-3 (PQ-1 + 6 ppm MAPD), MPS-4 (PQ-1 + 5 ppm MAPD + nonanoyl-EDTA), Per-1 (1-step hydrogen peroxide + platinum neutralizing disc) and PVI-1 (povidone iodine + neutralizing tablet). Ten Fusarium spp. (including F. solani ATCC 36031) were tested: 8 belonging to the F. solani species complex (FSSC) and 2 from the F. oxysporum species complex (FOSC). Fusarium strains were grown on potato dextrose agar in the dark for 10 days and the conidia purified and tested according to ISO 14729 with incubation times of up to 21 days.
MPS-1 and MPS-2 produced 3-5 Log kill with all strains after 6 hr exposure. MPS-3 gave a 2-4 Log kill at 6 hr for 8/10 strains and 0.6 -1.4 Log with the remaining two. MPS-4 showed 2-4 Log kill at 6 hr for 8/10 strains and 1.0 -1.4 Log for the others. Per-1 gave 1-3 Log kill for 4/10 strains at 6 hr and 0.1-0.8 Log for the rest (including ATCC 36031). PVI-1 gave 3-5 Log kill for seven strains tested by 4 hr, with no increased kill by 24 hr. Growth of Fusarium was not observed in any of the MPS’s over 21 days but did occur for Per-1 by 7 days with 3/10 strains (1-2 Log increase) and PVI-1 (2-4 Log increase) for 6/7 strains.
All MPS’s met the ISO 14729 criterion for stand-alone biocidal efficacy against F. solani (ATCC 36031). MPS-1 and MPS-2 showed greatest efficacy against all the strains compared to MPS-3 followed by MPS-4. Unlike MPS’s, contact lens disinfection systems that employ a neutralization step do not have continued antimicrobial presence to prevent surviving organism growth during storage. The findings of this study indicate that contact lenses should not to be stored for prolonged periods in a neutralized care system and, if so, ought to be disinfected before use.
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