March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Anti-inflammatory Effects Of Glucocorticoids On Endotoxin-induced Uveitis In Rats : Effects Of The Mode Of Administration
Author Affiliations & Notes
  • Pierre-Paul Elena
    Iris Pharma, La Gaude, France
  • Nicolas CIMBOLINI
    Iris Pharma, La Gaude, France
  • Sophie ANTONELLI
    Iris Pharma, La Gaude, France
  • Hélène DUBOS
    Iris Pharma, La Gaude, France
  • Laurence FERAILLE
    Iris Pharma, La Gaude, France
  • Philippe MARGARON
    Iris Pharma, La Gaude, France
  • Footnotes
    Commercial Relationships  Pierre-Paul Elena, IRIS PHARMA (E); Nicolas Cimbolini, IRIS PHARMA (E); Sophie Antonelli, IRIS PHARMA (E); Hélène Dubos, IRIS PHARMA (E); Laurence Feraille, IRIS PHARMA (E); Philippe Margaron, IRIS PHARMA (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6285. doi:
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      Pierre-Paul Elena, Nicolas CIMBOLINI, Sophie ANTONELLI, Hélène DUBOS, Laurence FERAILLE, Philippe MARGARON; Anti-inflammatory Effects Of Glucocorticoids On Endotoxin-induced Uveitis In Rats : Effects Of The Mode Of Administration. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6285.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : This study examined the outcomes of glucocorticoids administered topically, systemically, or subconjunctivally on endotoxin-induced uveitis (EIU) in rats.

Methods: : EIU was induced in male Lewis rats by footpad injection of lipopolysaccharide (LPS, 200 µg). Animals were randomized in four groups. The first two groups received either a single intravenous dose of 2.5 mg/kg dexamethasone phosphate (immediately after LPS inoculation) or multiple instillations of 0.1% dexamethasone (1h before and 1h, 2h and 3h after induction). The third group received a single subconjunctival dose of 1 µg methylprednisolone. A non-treated induced group was used as control of induction. Treatment effects were evaluated 24h after induction using clinical scoring, leucocyte and protein infiltration and cytokine production using multiplex analysis in aqueous humor (AH).

Results: : Both intravenous and topical administrations of dexamethasone markedly decreased clinical signs of EIU, inflammatory cell counts, protein concentration, and levels of IL1beta, TNFalpha, IL6 and IL12 in AH. Sub-conjunctival administration of methylprednisolone also decreased the symptoms of EIU but to a lesser extent than dexamethasone.

Conclusions: : In conclusion topical administration of dexamethasone allows for a therapeutic effect on the anterior segment of the eye in the rat EIU model, and may present a viable alternative to systemic administration of glucocorticoids.

Keywords: uveitis-clinical/animal model • cytokines/chemokines • corticosteroids 

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