March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Color Contrast Sensitivity Estimated With Two Different Psychophysical Methods
Author Affiliations & Notes
  • Luiz Carlos L. Silveira
    Nucleo de Medicina Tropical,
    Universidade Federal do Para, Belem, Brazil
  • Chanel D. Perry
    Biology, Biology Xavier University of Louisiana, New Orleans, Louisiana
  • Eliza Maria C. Lacerda
    Instituto de Ciencias Biologicas,
    Universidade Federal do Para, Belem, Brazil
  • Mellina M. Jacob
    Instituto de Ciencias Biologicas,
    Universidade Federal do Para, Belem, Brazil
  • Givago S. Souza
    Instituto de Ciencias Biologicas,
    Universidade Federal do Para, Belem, Brazil
  • Bruno D. Gomes
    Instituto de Ciencias Biologicas,
    Universidade Federal do Para, Belem, Brazil
  • Malinda E. Fitzgerald
    Anat & Neurobiol & Ophthalmol, UTHSC, Memphis, Tennessee
  • Footnotes
    Commercial Relationships  Luiz Carlos L. Silveira, None; Chanel D. Perry, None; Eliza Maria C. Lacerda, None; Mellina M. Jacob, None; Givago S. Souza, None; Bruno D. Gomes, None; Malinda E. Fitzgerald, None
  • Footnotes
    Support  CNPq-PRONEX/FAPESPA, NIH 5 T37 MD001378-11, CAPES, FINEP IBN-Net.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6395. doi:
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      Luiz Carlos L. Silveira, Chanel D. Perry, Eliza Maria C. Lacerda, Mellina M. Jacob, Givago S. Souza, Bruno D. Gomes, Malinda E. Fitzgerald; Color Contrast Sensitivity Estimated With Two Different Psychophysical Methods. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6395.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To compare color contrast sensitivities estimated by using two psychophysical methods with different criteria for threshold estimation.

Methods: : Six subjects (1 male, 6 females; 23.7 ± 1.9 years old) were studied; they were all normal trichromats with 20/20 corrected visual acuity. The subjects were monocularly tested using two methods for contrast threshold estimation: Mullen protocol (Mullen, 1985) and equiluminant stimulus protocol. Both protocols used a Gabor stimulus (12° standard deviation), generated along a red-green chromatic axis (CIE1976: red u’ = 0.288, v’= 0.480; green u’= 0.150, v’ 0.480) surrounded by the same mean chromaticity and luminance background (CIE1976: u’= 219, v’ = 0.480; 40 cd/m²). Eight spatial frequencies (0.1, 0.2, 0.4, 0.8, 1, 2, 4, and 8 cpd) color contrast (estimated as the chromaticity distance in the CIE1976 diagram) decreased according to a staircase of 12 reversals; contrast thresholds were taken as the mean contrast of the last 4 reversals. For the Mullen protocol, contrast threshold was estimated for 9 stimulus chromatic conditions and one achromatic condition. The color contrast threshold for each spatial frequency corresponded to the chromatic conditions that provided the largest difference in relation to the achromatic threshold. For the equiluminant stimulus protocol, a heterochromatic flicker photometry at 20 Hz was initially performed to find stimulus equiluminance condition before contrast threshold measurements. The chromaticity distance along the CIE1976 u’ axis was taken as the color contrast threshold for both protocols. One-way and two-way ANOVA and Bonferroni's HSD post-hoc test (α = 0.05), and Pearson’s correlation were used to compare the results obtained with the two protocols.

Results: : There were significant statistical differences for color contrast sensitivity estimated with the Mullen protocol between low spatial frequencies (0.1-0.8 cpd) and high spatial frequencies (8 cpd) (p < 0.01). There were significant statistical differences for color contrast sensitivity estimated with the equiluminant stimulus protocol between low spatial frequencies (0.2-1 cpd) and high spatial frequencies (8 cpd) (p < 0.01). There were no statistical differences for contrast sensitivity estimated with the two methods (p > 0.05).

Conclusions: : Spatial color contrast sensitivity estimated with two different psychophysical methods provided similar values for every spatial frequency tested. Color constancy at different luminance conditions has the potential to help the matching of color sensitivity obtained with very different psychophysical conditions.

Keywords: color vision • contrast sensitivity • color appearance/constancy 
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