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Shinichiro Ishikawa, Akira Hirata, Jo Nakabayashi, Ryo Iwakiri, Satoshi Okinami; Neuroprotective Effects Of Sirna, Targeted Caspase9, And Atelocollagen Complex On Rat Retinal Damage Induced By Transient Ischemic Injury. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6414.
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© ARVO (1962-2015); The Authors (2016-present)
Atelocollagen have an effect to extend small interfering RNA (siRNA) effect. In 2011 ARVO meeting, we reported caspase3 siRNA and atelocollagen complex protect the retinal ganglion cell damage induced by transient ischemic injury. We investigated whether the complex of atellocolagen and caspase9 siRNA, could protect retinal ganglion cells from apoptotic cell death by transient ischemic injury.
Seven week-old male Wister rats were used. We kept on rising IOP 110mmHg for 120 minutes then brought back to normal IOP. Atelocollagen and caspase9 siRNA complex (AC group) or caspase9 alone (C group) were injected into the vitreous cavity 24hr prior to transient ischemic injury. Atelocollagen and non-silencing siRNA complex were injected in eyes as control. Retinal ganglion cells were labeled with Fluoro-Gold retrogradely 3 day before transient ischemia. Seven days after the transient ischemia, flatmounts of the retina were examined by fluorescence microscopy.
In control group, the number of retinal ganglion cell was 584.1(+/-69.0) /mm2. In AC group, the number of ganglion cell was 599.1(+/- 25.6)/mm2 and in C groups, the number of retinal ganglion cell was 622.6(+/-89.2) /mm2. Between the AC group and control group, C group treated eyes showed no significant differences.
Atelocollagen and caspase9 siRNA complex showed less protective effects against retinal ganglion cell damage induced by transient ischemic injury, compared with that of atelocollagen and caspase3 siRNA complex.
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