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Yvonne Ou, Eric R. Lopez, David W. Sretavan, Erik M. Ullian; Retinal Ganglion Cell Synapse Loss In A Mouse Model Of Ocular Hypertension. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6595. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the effects of laser-induced ocular hypertension (LIOH) on retinal ganglion cell (RGC) synapse number in the retina and dorsal lateral geniculate nucleus (dLGN) of CD-1 albino mice.
Elevated intraocular pressure (IOP) was induced unilaterally using laser photocoagulation of the limbal and episcleral veins of adult CD-1 mouse eyes. IOP was measured by rebound tonometry. Retinal sections were immunostained with both pre-synaptic (e.g. ribeye) and post-synaptic (e.g. PSD95) markers to quantify synapse number in the inner plexiform layer. Synapse number in the dLGN was quantified using immunohistochemistry with vGluT2, an RGC pre-synaptic marker.
After laser-induced ocular hypertension, IOP elevations of the treated eye occurred within 6 hours and returned to baseline by 1 week. At early time points, there was loss of PSD95-positive puncta in the inner plexiform layer of the treated eye, but there was no difference in ribeye-positive puncta number or the number of Brn3a-positive RGCs in the ganglion cell layer. In the dLGN, there is loss of vGluT2-positive puncta in the contralateral dLGN, which fits our prediction given that the majority of retinogeniculate axons project to the contralateral dLGN.
RGC synapse loss occurs both in the inner plexiform layer of the retina and the contralateral dLGN in this laser-induced intraocular hypertension mouse model. The data supports further work focusing on RGC synapses as diagnostic and treatment targets.
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