March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Retinal Ganglion Cell Synapse Loss In A Mouse Model Of Ocular Hypertension
Author Affiliations & Notes
  • Yvonne Ou
    Ophthalmology,
    University of California, San Francisco, San Francisco, California
  • Eric R. Lopez
    Ophthalmology,
    University of California, San Francisco, San Francisco, California
  • David W. Sretavan
    Ophthalmology and Physiology,
    University of California, San Francisco, San Francisco, California
  • Erik M. Ullian
    Ophthalmology and Physiology,
    University of California, San Francisco, San Francisco, California
  • Footnotes
    Commercial Relationships  Yvonne Ou, None; Eric R. Lopez, None; David W. Sretavan, None; Erik M. Ullian, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6595. doi:https://doi.org/
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    • Get Citation

      Yvonne Ou, Eric R. Lopez, David W. Sretavan, Erik M. Ullian; Retinal Ganglion Cell Synapse Loss In A Mouse Model Of Ocular Hypertension. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6595. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine the effects of laser-induced ocular hypertension (LIOH) on retinal ganglion cell (RGC) synapse number in the retina and dorsal lateral geniculate nucleus (dLGN) of CD-1 albino mice.

Methods: : Elevated intraocular pressure (IOP) was induced unilaterally using laser photocoagulation of the limbal and episcleral veins of adult CD-1 mouse eyes. IOP was measured by rebound tonometry. Retinal sections were immunostained with both pre-synaptic (e.g. ribeye) and post-synaptic (e.g. PSD95) markers to quantify synapse number in the inner plexiform layer. Synapse number in the dLGN was quantified using immunohistochemistry with vGluT2, an RGC pre-synaptic marker.

Results: : After laser-induced ocular hypertension, IOP elevations of the treated eye occurred within 6 hours and returned to baseline by 1 week. At early time points, there was loss of PSD95-positive puncta in the inner plexiform layer of the treated eye, but there was no difference in ribeye-positive puncta number or the number of Brn3a-positive RGCs in the ganglion cell layer. In the dLGN, there is loss of vGluT2-positive puncta in the contralateral dLGN, which fits our prediction given that the majority of retinogeniculate axons project to the contralateral dLGN.

Conclusions: : RGC synapse loss occurs both in the inner plexiform layer of the retina and the contralateral dLGN in this laser-induced intraocular hypertension mouse model. The data supports further work focusing on RGC synapses as diagnostic and treatment targets.

Keywords: synapse • ganglion cells • thalamus/lateral geniculate nucleus 
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