March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Changes in Regenerated Tissue around a Poly(vinylpyrrolidone) Hydrogel Accommodative Intraocular Lens in the Rabbit Eye
Author Affiliations & Notes
  • Kyoko Miura
    Product Development, IOL Group, Medical Operations Headquarters,
    NIDEK Co., Ltd., Gamagori, Japan
  • Yuko Shinohara
    Bioengineering Institute,
    NIDEK Co., Ltd., Gamagori, Japan
  • Satoshi Yokota
    Product Development, IOL Group, Medical Operations Headquarters,
    NIDEK Co., Ltd., Gamagori, Japan
  • Shinji Nagasaka
    Product Development, IOL Group, Medical Operations Headquarters,
    NIDEK Co., Ltd., Gamagori, Japan
  • Hazuki Takahashi
    Product Development, IOL Group, Medical Operations Headquarters,
    NIDEK Co., Ltd., Gamagori, Japan
  • Masayoshi Nakatani
    Bioengineering Institute,
    NIDEK Co., Ltd., Gamagori, Japan
  • Tsutomu Sunada
    Product Development, IOL Group, Medical Operations Headquarters,
    NIDEK Co., Ltd., Gamagori, Japan
  • Footnotes
    Commercial Relationships  Kyoko Miura, None; Yuko Shinohara, None; Satoshi Yokota, None; Shinji Nagasaka, None; Hazuki Takahashi, None; Masayoshi Nakatani, None; Tsutomu Sunada, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6678. doi:
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      Kyoko Miura, Yuko Shinohara, Satoshi Yokota, Shinji Nagasaka, Hazuki Takahashi, Masayoshi Nakatani, Tsutomu Sunada; Changes in Regenerated Tissue around a Poly(vinylpyrrolidone) Hydrogel Accommodative Intraocular Lens in the Rabbit Eye. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6678.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : In a previous study in which we implanted a poly(vinylpyrrolidone) (PVP) hydrogel into the capsular bag of the rabbit eye, we reported that posterior capsule opacification (PCO) gradually developed by 3 months postoperatively; the capsule became transparent between 6 and 12 months postoperatively (i.e., ARVO 2008). One month postoperatively, the structure of the regenerated lens tissue around the PVP hydrogel was similar to that of a normal lens (i.e., ARVO 2009). Here, we examined the histological and ultrastructural alterations in the regenerated tissue 6 months postoperatively to elucidate the changes in PCO.

Methods: : PVP hydrogel was implanted into the capsular bags of Japanese white rabbits. Slitlamp examination was performed once monthly for 6 months postoperatively, and the rabbits were euthanized at postoperative month 6. The eyes were enucleated and the anterior segments were examined histologically under light and transmission electron microscopy.

Results: : Slitlamp examination: PCO decreased gradually from 5 months postoperatively, although a proliferation of opacified cells remained between the PVP hydrogel and the posterior capsule 6 months postoperatively. Light microscopy: At 6 months postoperatively, leucocytes and bladder cells, which were not found in the first postoperative month, were observed focally in the regenerated tissue between the hydrogel and the posterior capsule. Transmission electron microscopy: At 6 months, the cells beneath the anterior capsule had degenerated, and collagen fiber-like structures and several kinds of cell organelles remained. In the equatorial region many granules were present, but neither nuclei nor other organelles were observed. Many organelles and granules were observed along the periphery of the posterior capsule. A lens-fiber-like arrangement of adjacent cells was observed near the center of the cortex. However, the ball-and-socket junctions observed 1 month postoperatively were not found.

Conclusions: : These results indicated that the structure of the regenerated lens tissue around the PVP hydrogel had degenerated by 6 months postoperatively. This degeneration likely was related to the transparency of the PCO.

Keywords: posterior capsular opacification (PCO) • intraocular lens • microscopy: electron microscopy 
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