March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Measurement of retinal blood flow using dual beam bi-directional Fourier domain Doppler OCT - comparison with laser Doppler velocimetry
Author Affiliations & Notes
  • Rene M. Werkmeister
    Med Physics and Biomed Eng,
    Medical University of Vienna, Vienna, Austria
  • Nikolaus Dragostinoff
    Med Physics and Biomed Eng,
    Medical University of Vienna, Vienna, Austria
  • Stefan Palkovits
    Clinical Pharmacology,
    Medical University of Vienna, Vienna, Austria
  • Reinhard Told
    Clinical Pharmacology,
    Medical University of Vienna, Vienna, Austria
  • Leopold Schmetterer
    Clinical Pharmacology,
    Medical University of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships  Rene M. Werkmeister, None; Nikolaus Dragostinoff, None; Stefan Palkovits, None; Reinhard Told, None; Leopold Schmetterer, None
  • Footnotes
    Support  FWF P21570
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6828. doi:
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      Rene M. Werkmeister, Nikolaus Dragostinoff, Stefan Palkovits, Reinhard Told, Leopold Schmetterer; Measurement of retinal blood flow using dual beam bi-directional Fourier domain Doppler OCT - comparison with laser Doppler velocimetry. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6828.

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Abstract

Purpose: : We have recently introduced a technique for the measurement of retinal blood flow using bi-directional Fourier domain Doppler OCT. We have carefully validated this system by showing that blood flow is preserved at vessel bifurcations when measured with our system.

Methods: : In the present study we set out to investigate the response of retinal blood flow to 100% oxygen breathing. This was done in a group of 10 healthy subjects. Retinal blood velocities were measured on one day using bi-directional Laser Doppler Velocimetry (LDV). On another study day retinal blood velocities were measured using bi-directional Fourier domain Doppler OCT. On both days retinal vessel diameters were measured using the Imedos Retinal Vessel Analyzer.

Results: : 100% oxygen breathing caused a highly significant decrease in blood velocities, vessel diameters and blood flow (p < 0.01 each). The data obtained on the 2 study days showed a high degree of correlation (p > 0.05 each), and the amount of blood flow reduction was in good agreement between the two methods employed.

Conclusions: : Our data confirm our previous findings that bi-directional Fourier domain Doppler OCT is a valid method for assessing retinal blood flow. The results we obtained with this technique are in good agreement with previously published data using other techniques.

Keywords: blood supply • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • image processing 
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