March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Evaluation of Ultrasound-Assisted Thrombolysis Using Nontargeted Ultrasound Contrast Agents in a Model of Retinal Vein Occlusion
Author Affiliations & Notes
  • Walid F. Abdallah
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Ophthalmology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Hitenkumar Patel
    Radiology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • Edward Grant
    Radiology, Keck School of Medicine, University of Southern California, Los Angeles, California
  • Gerald J. Chader
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Mark S. Humayun
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
  • Footnotes
    Commercial Relationships  Walid F. Abdallah, None; Hitenkumar Patel, None; Edward Grant, None; Gerald J. Chader, 20080262512 (P); Mark S. Humayun, 20080262512 (P)
  • Footnotes
    Support  NEI core grant EY03040
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6830. doi:https://doi.org/
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      Walid F. Abdallah, Hitenkumar Patel, Edward Grant, Gerald J. Chader, Mark S. Humayun; Evaluation of Ultrasound-Assisted Thrombolysis Using Nontargeted Ultrasound Contrast Agents in a Model of Retinal Vein Occlusion. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6830. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To study the potential efficacy of ultrasound (US)-assisted microbubble (MB) destruction as an innovative thrombolytic tool for the treatment of retinal vein occlusion (RVO).

 
Methods:
 

Experimental RVO in the right eyes of 40 rabbits was induced using laser photothrombosis followed 48 hs later by the US experiment. Rabbits were randomly divided into 4 equal groups: US+MB group, US+saline group, MB+sham US group, and no treatment group. The last 3 groups acted as control. Fluorescein fundus angiography (FFA) and pulse Doppler US were used to evaluate the retinal blood flow. Pulse Doppler measurements were represented as mean ± SD and one-way ANOVA test was used to compare means. Statistical significance was defined as P <0.05.

 
Results:
 

MB-assisted US thrombolysis resulted in restoration of flow in 70% of rabbits. (Fig.1) None of the control groups showed significant restoration of retinal venous blood flow. The mean venous blood velocity for the US+MB group improved from 0.05±0.1 cm/s before the experiment to 1.28±0.9 cm/s at the end of the experiment with a statistically significant difference (p value = 0.002). On the other hand, the final measurements of retinal venous blood velocity for the 3 control groups were not statistically different from the baseline i.e. postlaser measurements. (Fig.2) Pulse Doppler US measurement of the retinal blood velocity in retinal vessels correlated well with the FFA results.

 
Conclusions:
 

US-assisted thrombolysis using non-targeted contrast agents is a promising therapeutic tool for RVO and may serve as a new, noninvasive intervention for RVO in humans.  

 

 
Keywords: vascular occlusion/vascular occlusive disease • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina 
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