March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Effect of Slow Releasing Hydrogen Sulfide Donor GYY4137 on Isolated Bovine Ciliary Artery
Author Affiliations & Notes
  • Madhura S. Kulkarni
    Pharmaceutical Sciences,
    Texas Southern University, Houston, Texas
  • Ya Fatou Njie-Mbye
    Pharmaceutical Sciences,
    Texas Southern University, Houston, Texas
  • Catherine A. Opere
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • Matt Whiteman
    University of Exeter, Peninsula Medical School, Exeter, United Kingdom
  • Sunny E. Ohia
    Provost/Academic Affairs,
    Texas Southern University, Houston, Texas
  • Footnotes
    Commercial Relationships  Madhura S. Kulkarni, None; Ya Fatou Njie-Mbye, None; Catherine A. Opere, None; Matt Whiteman, None; Sunny E. Ohia, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 6866. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Madhura S. Kulkarni, Ya Fatou Njie-Mbye, Catherine A. Opere, Matt Whiteman, Sunny E. Ohia; Effect of Slow Releasing Hydrogen Sulfide Donor GYY4137 on Isolated Bovine Ciliary Artery. Invest. Ophthalmol. Vis. Sci. 2012;53(14):6866.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Hydrogen sulfide (H2S), a colorless gas characterized by its pungent odor of rotten eggs has been reported to elicit relaxation effects on non-ocular smooth muscles of several mammalian species. The purpose of the present study is to investigate whether the slow releasing H2S donor, GYY4137 can induce pharmacological actions on isolated bovine ciliary artery.

Methods: : Isolated bovine posterior ciliary arteries were set up in 25 mL organ baths containing oxygenated Krebs solution (pH 7.4) at 370 C. Changes in longitudinal isometric tension were recorded via a grass FT03 Force-displacement Transducers and analyzed using the Grass PolyView computer software. The relaxant action of GYY 4137 on phenylephrine induced tone was studied in the absence or presence of inhibitors of enzymes for the biosynthetic pathways of H2S and prostanoids. In addition, we examined the effects of ATP-sensitive K+ (KATP) channel antagonist, glibenclamide on GYY4137 induced response.

Results: : The slow releasing H2S donor, GYY4137 (10nM-0.1µM) elicited a concentration-dependent relaxation of phenylephrine induced tone in bovine ciliary artery. This response was significantly (P<0.05) enhanced in the presence of the cyclo-oxygenase inhibitor, flurbiprofen (3μM). Other H2S donors (MC05, MC06 and sodium hydrosulfide (NaHS) also exerted similar response in the following order of magnitude; MC06>NaHS>GYY4137>MC05. Interestingly, the inhibitor of cystathionine β-synthase, AOA (30µM) caused a rightward shift in the concentration-response curve to GYY4137 whilst, the inhibitor of cystathionine γ-lyase, PAG (1mM) only attenuated relaxations caused by high concentrations of GYY4137 (>1µM). The KATP channel antagonist, glibenclamide (100µM) did not affect the relaxation action induced by GYY4137 on ciliary artery.

Conclusions: : The slow releasing H2S donor, GYY4137 can relax isolated bovine ciliary artery, an effect dependant on endogenous production of H2S. Prostanoids are involved in the inhibitory action of GYY4137. The observed vascular relaxation induced by GYY 4137 suggest that slow releasing H2S molecules might serve as potential therapeutic agents to increase ocular blood flow, consequently preserving the optic nerve and alleviating vision loss.

Keywords: neurotransmitters/neurotransmitter systems • enzymes/enzyme inhibitors • blood supply 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.