April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Differential Profiles of MicroRNAs in Retinoblastoma Cell Lines of Different Proliferation and Adherence Patterns
Author Affiliations & Notes
  • Dong Hyun Jo
    Fight against Angiogenesis-Related Blindness (FARB) laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Department of Ophthalmology, College of Medicine,
    Seoul National University, Seoul, Republic of Korea
  • Jin Hyoung Kim
    Fight against Angiogenesis-Related Blindness (FARB) laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
  • Hyun Beom Song
    Fight against Angiogenesis-Related Blindness (FARB) laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Department of Ophthalmology, College of Medicine,
    Seoul National University, Seoul, Republic of Korea
  • Woong-Yang Park
    Department of Biomedical Sciences, College of Medicine,
    Seoul National University, Seoul, Republic of Korea
  • Young Suk Yu
    Fight against Angiogenesis-Related Blindness (FARB) laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Department of Ophthalmology, College of Medicine,
    Seoul National University, Seoul, Republic of Korea
  • Jeong Hun Kim
    Fight against Angiogenesis-Related Blindness (FARB) laboratory, Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Republic of Korea
    Department of Ophthalmology, College of Medicine,
    Seoul National University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Dong Hyun Jo, None; Jin Hyoung Kim, None; Hyun Beom Song, None; Woong-Yang Park, None; Young Suk Yu, None; Jeong Hun Kim, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2481. doi:
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      Dong Hyun Jo, Jin Hyoung Kim, Hyun Beom Song, Woong-Yang Park, Young Suk Yu, Jeong Hun Kim; Differential Profiles of MicroRNAs in Retinoblastoma Cell Lines of Different Proliferation and Adherence Patterns. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2481.

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Abstract

Purpose: : To investigate differential expression of microRNAs (miRNAs) in retinoblastoma cell lines of different growth patterns: SNUOT-Rb1 with adherent and more rapid growth and Y79 with nonadherent and slower growth

Methods: : MiRNAs were isolated from 2 different human retinoblastoma cell lines: SNUOT-Rb1 and Y79. Using the miRNA microarray, differentially expressed miRNAs between 2 cell lines were analyzed out. Common mRNA targets of each miRNA were extracted from 4 web-based databases: TargetScan, miRanda, RNAhybrid, and PicTar. Biological functions of target genes were predicted with the PANTHER Classification System.

Results: : We identified 39 differentially expressed miRNAs between 2 cell lines: 22 were upreglated in SNUOT-Rb1 cells, and the other 17 were overexpressed in Y79 cells. More than half of top 10 mRNA targets of hsa-miR-10b, hsa-miR-29a, hsa-miR-29b, hsa-miR-29c, and hsa-let-7c in SNUOT-Rb1 cells and hsa-miR-34a, hsa-miR-34c-5p, hsa-miR-124, hsa-miR-135b, hsa-miR-142-5p, and hsa-let-7i in Y79 cells were related with biological processes which could affect the growth patterns of cells: cell adhesion, cell cycle, cell death, and cell division.

Conclusions: : Differential miRNA profiles from 2 retinoblastoma cell lines may reflect the growth patterns of them. Based on the data from the target analysis of each miRNA, we found out several miRNAs which were differentially expressed had targets of possible impact on progression of the RB. From these analyses, we suggest that some differential miRNAs could have roles in miRNA-targeted treatments on the retinoblastoma.

Keywords: retinoblastoma • proliferation • cell adhesions/cell junctions 
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