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Tai-ichiro Chikama, Ji-Ae Ko, Koh-hei Sonoda; Up-regulation Of IL-6 Released From Corneal Fibroblasts Cocultured With Neural Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2521.
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Neural regulation plays an important role for maintenance of corneal transparency. In the cases of neurotrophic keratopathy, severe visual disturbance can occur with delayed epithelial resurfacing and transition to ulceration. The corneal stroma consists essentially networks of keratocytes and nerve fibers in collagen lamellae. In this study we examined the effect of neural cells on the behavior of corneal fibroblasts with a coculture system.
Human corneal fibroblast (HCF) and established neural cells (PC12) were cultured on opposite sides of a collagen vitrigel membrane in serum free media. A multiplex bead-based arrays was used to determine 10 cytokines including interleukin 1β (IL-1β),interleukin 1 receptor antagonist (IL-1ra),interleukin 4(IL-4),interleukin 6 (IL-6), interleukin 8 (IL-8),interleukin 13 (IL-13), eotaxin, basic FGF, tumor necrosis factor α (TNFα) and vascular endothelial growth factor (VEGF) in culture media.
The presence of neural cells synergistically promoted four times higher released amounts of IL-6 from HCF compared with HCF alone. The other cytokines were not changed by coculture with neural cells.
Since IL-6 is known to facilitate corneal epithelial cell migration, these results suggest that known or unknown factor(s) released from neural cells may promote corneal epithelial wound healing via IL-6 secretion from HCF.
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