April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Neuroprotective Effect Of AAV Mediated BDNF On Retinal Ganglion Cells Survival Of Experimental Glaucoma DBA/2J Mice
Author Affiliations & Notes
  • Chukai Huang
    Joint Shantou Int'l Eye Center, Shantou, China
  • Lifang Liu
    Joint Shantou Int'l Eye Center, Shantou, China
  • Linping Cen
    Joint Shantou Int'l Eye Center, Shantou, China
  • Miaomiao Han
    Joint Shantou Int'l Eye Center, Shantou, China
  • Mingzhi Zhang
    Joint Shantou Int'l Eye Center, Shantou, China
  • Footnotes
    Commercial Relationships  Chukai Huang, None; Lifang Liu, None; Linping Cen, None; Miaomiao Han, None; Mingzhi Zhang, None
  • Footnotes
    Support  National Natural Science Foundation of China (30672282)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3081. doi:
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      Chukai Huang, Lifang Liu, Linping Cen, Miaomiao Han, Mingzhi Zhang; The Neuroprotective Effect Of AAV Mediated BDNF On Retinal Ganglion Cells Survival Of Experimental Glaucoma DBA/2J Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3081.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the neuroprotective effect of AAV mediated BDNF on retinal ganglion cells survival of experimental glaucoma DBA/2J mice

Methods: : Totally 10 DBA/2J mice were used for intravitreal injection with 1 microliter AAV-BDNF-GFP (n=10) in the left eyes at the age of 6 months old, while the right eyes were injected with same volume saline. IOP was measured by Tonolab in mouse model every month. 3 months after injection,retinas were taken out for investigating GFP positive RGCs under fluorescence microscopy. Immunohistochemistry staining was done by applying TUJ1 and cy3 to identify survival RGCs.

Results: : The IOP of DBA/2J mice was 11.71mmHg and 11.50mmHg for the right eyes and left eyes respectively at 4 months old. From 5 months on,the IOP of DBA/2J mice raised, and reached peak when 7 months old. There was no significant statistical difference between the right and the left eye during 4 months to 9 months old(t=0.82,P =0.45 ). Three months after vitrous injection of AAV-BDNF-GFP, retinas showed GFP positive cells significantly. BDNF over-expression leaded to more RGCs survival than the control eyes(3168.13±1319.33/ mm2 & 2024.81±796.38/ mm2, t=2.75,P =0.02).

Conclusions: : These data suggest that BDNF can exert a protective effect in DBA/2J glaucoma mice.

Keywords: neuroprotection • ganglion cells • gene transfer/gene therapy 
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