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Chukai Huang, Lifang Liu, Linping Cen, Miaomiao Han, Mingzhi Zhang; The Neuroprotective Effect Of AAV Mediated BDNF On Retinal Ganglion Cells Survival Of Experimental Glaucoma DBA/2J Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3081.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the neuroprotective effect of AAV mediated BDNF on retinal ganglion cells survival of experimental glaucoma DBA/2J mice
Totally 10 DBA/2J mice were used for intravitreal injection with 1 microliter AAV-BDNF-GFP (n=10) in the left eyes at the age of 6 months old, while the right eyes were injected with same volume saline. IOP was measured by Tonolab in mouse model every month. 3 months after injection,retinas were taken out for investigating GFP positive RGCs under fluorescence microscopy. Immunohistochemistry staining was done by applying TUJ1 and cy3 to identify survival RGCs.
The IOP of DBA/2J mice was 11.71mmHg and 11.50mmHg for the right eyes and left eyes respectively at 4 months old. From 5 months on,the IOP of DBA/2J mice raised, and reached peak when 7 months old. There was no significant statistical difference between the right and the left eye during 4 months to 9 months old(t=0.82,P =0.45 ). Three months after vitrous injection of AAV-BDNF-GFP, retinas showed GFP positive cells significantly. BDNF over-expression leaded to more RGCs survival than the control eyes(3168.13±1319.33/ mm2 & 2024.81±796.38/ mm2, t=2.75,P =0.02).
These data suggest that BDNF can exert a protective effect in DBA/2J glaucoma mice.
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