April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Increase In Mitochondrial DNA Mutations Impairs Retinal Function And Increase Retinal Ganglion Cell Susceptibility To Injury
Author Affiliations & Notes
  • Yu Xiang George Kong
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Ian A. Trounce
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Bang V. Bui
    Optometry & Vision Sciences, University of Melbourne, Melbourne, Australia
  • Nicole Van Bergen
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Vicki Chrysostomou
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Hayley Waugh
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Algis J. Vingrys
    Optometry & Vision Sciences, University of Melbourne, Melbourne, Australia
  • Jonathan G. Crowston
    University of Melbourne, Center for Eye Research Australia, Melbourne, Australia
  • Footnotes
    Commercial Relationships  Yu Xiang George Kong, None; Ian A. Trounce, None; Bang V. Bui, None; Nicole Van Bergen, None; Vicki Chrysostomou, None; Hayley Waugh, None; Algis J. Vingrys, None; Jonathan G. Crowston, None
  • Footnotes
    Support  National Health and Medical Research Council, Ophthalmic Research Institute of Australia Grants, Glaucoma Australia Fund, Henry Greenfield Research Fund, Edols Trust Fund, Victorian Government
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3090. doi:
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      Yu Xiang George Kong, Ian A. Trounce, Bang V. Bui, Nicole Van Bergen, Vicki Chrysostomou, Hayley Waugh, Algis J. Vingrys, Jonathan G. Crowston; Increase In Mitochondrial DNA Mutations Impairs Retinal Function And Increase Retinal Ganglion Cell Susceptibility To Injury. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3090.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract 
 
Purpose:
 

Acquired mitochondrial DNA (mtDNA) mutations in neurons and other tissues are a hallmark of biological aging. This study determined whether mtDNA mutations impact on retinal ganglion cell (RGC) function and the RGC response to intraocular pressure (IOP) elevation.

 
Methods:
 

We employed a transgenic mouse model with a neural-targeted proof-reading defect in mitochondrial polymerase gamma that acquires mtDNA mutations at a faster rate than normal. Transgenic (POLG) and wildtype littermate (WT) at 3 and 12 months of age were dark adapted for measurement of baseline retinal function using the full-field scotopic electroretinogram (ERG). Retinal response to progressive IOP elevation (12 - 80 mmHg) via anterior chamber cannulation was examined on 12-month old POLG and WT mice. ERG was analyzed in terms of retinal ganglion cell (positive scotopic threshold response, pSTR), ON-bipolar cell (b-wave) and photoreceptor (a-wave) responses. Statistical comparisons were performed using Student’s t-test.

 
Results:
 

Three month old POLG mice(n=7) showed no differences in retinal response compared with controls (n=10). However at 12 months of age, POLG mice (n=13) showed 43±9% reduction in pSTR amplitude (RGC,P<0.01) and a 29±8% reduction in b-wave amplitude (bipolar cells, P<0.05) compared with controls(n=8), despite having normal a-wave. POLG mice also showed increased susceptibility to IOP elevation(Figure); IOP required to cause 50% reduction in pSTR was significantly lower for POLG mice (31 mmHg,95% CI[27,34]) than controls (42 mmHg,95% CI[38,46]). These changes correlated with mtDNA mutations and reduced mitochondrial enzyme activity (by 36±9%) in the retina.

 
Conclusions:
 

The accumulation of mtDNA mutations leads to accelerated loss of retinal function and reduced capacity to withstand IOP stress, providing a potential mechanism whereby increasing age predisposes to glaucoma.  

 
Keywords: aging • mitochondria • ganglion cells 
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