April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
β-adrenergic Antagonists, Carbonic Anhydrase Inhibitors And α2-agonists Reduce The Effects Of Cannabinoids In A Rat Glaucoma Model
Author Affiliations & Notes
  • Kevin M. Bowman
    Ophthalmology, T.R. Lee Center for Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Sandeep S. Samudre
    Physiological Sciences,
    T.R. Lee Center For Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Ben Mandel
    Ophthalmology,
    T.R. Lee Center For Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Alireza Hosseini
    Physiological Sciences,
    T.R. Lee Center For Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Patricia B. Williams
    Physiological Sciences,
    T.R. Lee Center For Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Frank A. Lattanzio, Jr.
    Physiological Sciences,
    T.R. Lee Center For Ocular Pharmacology, Eastern Virginia Medical School, Norfolk, Virginia
  • Footnotes
    Commercial Relationships  Kevin M. Bowman, None; Sandeep S. Samudre, None; Ben Mandel, None; Alireza Hosseini, None; Patricia B. Williams, None; Frank A. Lattanzio, Jr., None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3101. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kevin M. Bowman, Sandeep S. Samudre, Ben Mandel, Alireza Hosseini, Patricia B. Williams, Frank A. Lattanzio, Jr.; β-adrenergic Antagonists, Carbonic Anhydrase Inhibitors And α2-agonists Reduce The Effects Of Cannabinoids In A Rat Glaucoma Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3101.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

Topically applied endocannabinoid analogs have been reported to reduce intraocular pressure (IOP). Combination of timolol, a β-adrenergic antagonist and dorzolamide, a carbonic anhydrase inhibitor, decreases IOP more than either drug alone. In this study, the endocannabinoid analog, O-1812, was combined with timolol, dorzolamide, or brimonidine, an α2-agonist, to evaluate the effect of the combination on IOP in a rat glaucoma model.

 
Methods:
 

Surgical ligation of 3 of the 4 episcleral veins in eyes of Sprague Dawley rats caused a lasting IOP increase. IOP was measured by Tonolab at baseline and at 30, 60 and 120 min. Each drug was applied alone or in combination with O-1812. For combination therapy, following baseline IOP, timolol 0.5%, brimonidine 0.2% or dorzolamide 2.0% were applied topically followed 10 min later by O-1812 1.0%. Analysis for ocular irritation was performed at slit lamp at baseline and at 120 min. Results are reported as a percentage of baseline.

 
Results:
 

Within 30 min all drugs and combinations significantly decreased IOP compared to baseline (p<0.05). Among single drug regimens O-1812 and dorzolamide provided the largest reduction (p<0.05). Yet, when O-1812 was combined with timolol or dorzolamide, IOP was significantly greater than either drug alone (p<0.05). When O-1812 was combined with brimonidine, reduction in IOP was greater than brimonidine alone, but not different from O-1812 alone. No drug alone or in combination caused ocular irritation or systemic effects.

 
Conclusions:
 

Combination of cannabinoids with β-adrenergic antagonist or carbonic anhydrase inhibitor reduced the effect of cannabinoid. Although cannabinoids reduce IOP primarily through CB1 receptor, it could be postulated that further downstream signalling events depend on cross reactivity between β-adrenergic receptors and carbonic anhydrase inhibitors.  

 
Keywords: intraocular pressure 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×