April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Effect Of Prostaglandin Analogues On Inhibition Of Adipocytes Differentiation And Adipogenesis
Author Affiliations & Notes
  • Zhuxi Wang
    department of ophthalmolology, University of Tokyo Hospital, Tokyo, Japan
  • Reiko Yamagishi
    department of ophthalmolology, University of Tokyo Hospital, Tokyo, Japan
  • Takashi Fujishiro
    Saitama Red Cross Hospital, Saitama, Japan
  • Makoto Aihara
    department of ophthalmolology, University of Tokyo Hospital, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Zhuxi Wang, None; Reiko Yamagishi, None; Takashi Fujishiro, None; Makoto Aihara, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3102. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Zhuxi Wang, Reiko Yamagishi, Takashi Fujishiro, Makoto Aihara; Effect Of Prostaglandin Analogues On Inhibition Of Adipocytes Differentiation And Adipogenesis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3102.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Prostaglandin analogues (PGs) are widely used for anti-glaucoma therapy. Recently, deepening of upper eyelid sulcus (DUES) are reported as cosmetic side effects in bimatoprost and travoprost, but not in latanoprost. However, the mechanism of DUES is unclear. We hypothesized orbital fat reduction is one of the causes of DUES, and planned to study the effect of PGs on adipogenesis in vitro.

Methods: : 3T3-L1 preadipocytes were cultured and differentiated into adipocytes (day 0). At day 2, unoprostone (UNO), latanoprost acid (LAT-A), travoprost acid (TRA-A), tafluprost acid (TAF-A), bimatoprost (BIM), bimatoprost acid (BIM-A), and prostaglandin F2a (PGF2a) were added at the concentration of 100nM. At day 10, intracellular oil stained with Oil Red O were photographed by a microscopy to measure the area of oil. In one assay using all drugs, 50 areas were counted and 5 dependent experiments were repeated in a masked manner. The relative area of oil in the treated culture was calculated in comparison with that in the control culture with DMSO vehicle solution and analyzed by Dunnet test.

Results: : The relative oil area of LAT-A, TRA-A, TAF-A, BIM, BIM-A, UNO and PGF2a were 35.8±18.7, 25.8±11.7, 41.2±22.3, 112.7±61.0, 17.9±9.0, 100.9±43.2, and 45.2±38.0%, respectively. All acid form of prost-type PGs, LAT-A, TRA-A, TAF-A, BIM-A, and PGF2a inhibited adipogenesis. TRA-A and BIM-A significantly inhibited adipogenesis (p<0.05), but BIM and UNO did not. Prost-type PGs with high FP receptor affinity tended to show strong inhibitory effect compared to prostamide-type BIM and prostone-type UNO.

Conclusions: : Although DUES were not clarified in all PGs, all acid forms of prost-type FP agonists were more potent than BIM and UNO in interfering adipogenesis in vitro. This result suggests that all prost-type PG analogues may have a potential to induce DUES, probably due to orbital fat reduction.

Keywords: lipids • pathology: experimental • differentiation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×