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Heloisa H. Russ, Nubia V. Faria, Paulo A. Mello, Fabiano Montiani-Ferreira, Palloma Rose, Sebastião Cronemberger, Lucia Noronha; Conjunctival Changes Induced by Prostaglandin Analogues, Timolol Maleate and Fixed Combinations of Prostaglandin Analogues + Timolol Maleate: A Comparative Histomorphometric and Immunohistochemical Study. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3111.
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© ARVO (1962-2015); The Authors (2016-present)
To compare histological and immunohistochemical (IHC) changes induced by prostaglandin analogues, timolol maleate and fixed combinations on rabbit conjunctiva.
70 New Zealand rabbits were divided in 7 groups of 10 animals. The left eyes were treated with one daily drop of one of the drugs: bimatoprost 0.03%, travoprost 0.004%,latanoprost 0.005%,timolol maleate 0.5%, timolol maleate 0.5%+bimatoprost 0.03% ,timolol maleate 0.5%+travoprost 0.004%and timolol maleate 0.5%+latanoprost 0.005% for 30 days. Following euthanasia and enucleation, the eyes were routinely processed and stained with HE and for IHC using antibodies anti-RAM 11, anti-CD45, CD74, VCAM. Parameters evaluated were: inflamatory reaction, epithelial thickness, number of vessels, vascular diameter and number of goblet cells. One-way ANOVA with a significance level of 5% followed by Tukey-Kramer post hoc tests were used to analyze all numerical variables.
All groups of fixed combination presented a significant increase of the inflammatory cell compared to respective isolated prostaglandin: All of them had increase on epithelial thickness and in the number of blood vessels.Ganfort induced an increase in the number of goblet cells compared to Lumigan (p=0.025). The IHQ results showed significant increase of CD45 in all drugs and VCAM increased in G/L (p<0.0001) and XC/X (p=0.04). Duo-travatan compared to Travatan induced increase of expression of RAM 11 (p=0.0002). None of them induced expression of CD74. Comparing FC to timolol, Duotravatan and Ganfort induced increase of inflammatory cells.Xalacom increased epithelial thickness (p=0.0002) and vascular diameter (p=0.04). None of the drugs increased the expression of CD45, CD74 and RAM 11.
Comparing fixed combination to isolated prostaglandin analogues, almost all inflammatory parameters increased (epithelial thickness, inflammatory cells and number of blood vessels).
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