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Marcelo Zas; Retinal Stem Cells And Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3186.
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© ARVO (1962-2015); The Authors (2016-present)
To describe the cellular composition of epiretinal membranes from patients with several pathophysiological types of RD associated with PVR by immunostaining for retinal stem cells (RSC).
Nine patients including two myopic, three pseudophakic and four traumatic RD with PVR underwent PPV for resolution were analyzed. Average age was 59.22 years. Patients with PDR, intraocular tumors, posterior uveitis, retinal vascular disease and retinal dialysis were excluded. Membranes obtained were processed using the free floating protocol for immunofluorescence. GFAP was used because all the membranes are glial cells (astrocytes and retinal Müller cells). Nestin is a marker that identifies neural progenitor cells, as well as DAPI, which binds to DNA allowing identification of the nuclei. Confocal microscopy was also performed.
Main contribution was to identify RSC in epiretinal membranes of patients with PVR and try to establish its relationship with different pathophysiologic types of RD. In this series, three traumatic RDs expressed positive marks for Nestin, GFAP and DAPI. Two pseudophakic RDs were positive out of four cases. In the two myopic RDs retinal stem cell expression was negative. These results suggest that stem cells may have a relationship with the severity of the RD and PVR results, comparing the traumatic versus myopic and pseudophakic.
Epiretinal membranes of patients with PVR secondary to ocular trauma, either blunt or penetrating, expressed positive marks for RSC. No epiretinal membranes of patients with PVR secondary to myopic RD and only half of the cases of pseudophakic RD did express RSC. These results suggest that RSC would play a role on the extent or degree of trauma or injury and possibly the development of PVR.
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