April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Role Of Muscarinic Acetylcholine Receptors And Ca2+ Signaling In Tear Secretion
Author Affiliations & Notes
  • Takaaki Inaba
    Ophthalmology, Keio University, Shinjyuku-ku, Japan
  • Chihiro Hisatsune
    RIKEN BSI, wako-shi,saitama, Japan
  • Tetsuya Kawakita
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Yasumasa Sasaki
    Research & Dvelopment Center, Santen Pharmaceutical Co, Ltd, Ikoma-shi, Japan
  • Yoko Ogawa
    Department of Ophthalmology, Keio Univ School of Medicine, Shinjuku-Ku, Japan
  • katsuhiko mikoshiba
    RIKEN BSI, wako-shi, saitama, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  Takaaki Inaba, None; Chihiro Hisatsune, None; Tetsuya Kawakita, None; Yasumasa Sasaki, None; Yoko Ogawa, None; katsuhiko mikoshiba, None; Kazuo Tsubota, None
  • Footnotes
    Support  JSPS Grant Challenging Exploratory Research (No. 20659272)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3732. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Takaaki Inaba, Chihiro Hisatsune, Tetsuya Kawakita, Yasumasa Sasaki, Yoko Ogawa, katsuhiko mikoshiba, Kazuo Tsubota; Role Of Muscarinic Acetylcholine Receptors And Ca2+ Signaling In Tear Secretion. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3732.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Muscarinic acetylcholine receptor (mAchR) is a G-coupled receptor which consists of 5 distinct subtypes. However, relationship between tear secretion and subtypes of mAchR has not been reported. Therefore, we examined the role of mAchRs and Ca2+ signaling in tear secretion by using various mAchR genes knockout (mAchRKO) mice.

Methods: : Tear secretion was measured after treatment with 3 mg/kg pilocarpine. Pathological analyses were performed to evaluate lacrimal gland tissue sections with a light microscopy and an electron microscopy. In order to examine Ca2+ signaling induced by cholinergic stimulation in lacrimal gland, the Ca2+ in lacrimal gland acinar cells from mAchRKO mice and wild-type mice were measured ratiometrically using Ca2+-sensing dye (Fura-2).

Results: : Double deficient mice of M1 and M3 (M1/M3 AchRKO) genes showed significant decrease in pilocarpine-stimulated tear secretion compared with wild type mice. In addition, tear deficiency in M1/M3 AchRKO mice was caused by severely impaired Ca2+ signaling in the lacrimal gland acinar cells. Lacrimal gland dysfunction caused abnormal accumulation of secretory granules in the acinar epithelia.

Conclusions: : We concluded that M1/M3 AchRs and Ca2+ signaling are key molecules for tear secretion. Our findings suggest that M1/M3 AchR KO mice may become a novel dry eye model.

Keywords: lacrimal gland • cornea: tears/tear film/dry eye • acetylcholine 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.