April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Short term Grow and Viability in Lacrimal Gland Primary Culture
Author Affiliations & Notes
  • Leonardo T. Malki
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Jayter S. de Paula
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Ana C. Dias
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Carolina M. Módulo
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Mônica Alves
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Eduardo M. Rocha
    Ophthalmology, FMRP-USP, Ribeirao Preto, Brazil
  • Footnotes
    Commercial Relationships  Leonardo T. Malki, None; Jayter S. de Paula, None; Ana C. Dias, None; Carolina M. Módulo, None; Mônica Alves, None; Eduardo M. Rocha, None
  • Footnotes
    Support  CAPES FAPESP CNPq FAEPA
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3745. doi:
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      Leonardo T. Malki, Jayter S. de Paula, Ana C. Dias, Carolina M. Módulo, Mônica Alves, Eduardo M. Rocha; Short term Grow and Viability in Lacrimal Gland Primary Culture. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3745.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Cultures of acinar cells of lacrimal gland are considered an important method in understanding the mechanisms and pathways involved in the ocular surface diseases caused by dry eye. The aim of this study is to characterize the behavior of acinar cells as well as its functionality in a simplified method for culture of lacrimal gland. The evaluation included the number of cells, the viability and morphology by cells in culture.

Methods: : Acinar cells of the lacrimal gland of male Wistar rats were isolated with supplemented DMEM after being mechanically separated, enzymatic digested and filtered, and allowed sedimentation and transferred to plates with Matrigel. The number of cells, the total viability and the cell morphology were followed with Neubauer chamber and microscopy along 12 days.

Results: : Cell number increased until day 3 and started to decay thereafter. The viability was stable around 86.2 ± 1.05 %, until day 9, and at day 12 it reduced to 80.7 ± 1.73%. At the first hours cell shape were round and they tended to group by day 9.

Conclusions: : The present work presents morphological and functional aspects of a simplified method for culture of lacrimal gland acinar cells. The data show good viability and cell number in the first two weeks. These results are relevant for future ex vivo studies involving the pathophysiology of dry eye syndrome.

Keywords: cornea: tears/tear film/dry eye • lacrimal gland • extracellular matrix 
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