Purchase this article with an account.
Monica Alves, Lilian E. Costa Mendes da Silva, Stella F. de Freitas, Ana C. Dias, Leonardo T. Malki, Carolina M. Módulo, Jayter S. de Paula, Eduardo M. Rocha; Sex Difference in Innate Anti Microbial and House Keeping Proteins in Rat Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3746.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Several proteins are part of the innate anti microbial defense and several others are being used as endogenous biomarkers of exocrine gland function. However, their sexual differences and the expression of different peroxidases remain unclear . The aims of this study are 1) to compare the expression of house keeping proteins in lacrimal gland of male and female rats 2) to identify and distinguish the members of peroxidase superfamily in rat lacrimal gland (LG) of both genders.
Male and female Wistar rats lacrimal glands (LG) were homogenized and evaluated by Western blot (WB) to compare the expression of glutathione peroxidase-3 (GPX3), lactoperoxidase (LPO), lactoferrin and alpha-tubulin and beta actin. Total peroxidase activity was also compared among the groups after protein normalization (bradford method).
Western blot analysis had shown that GPX3, LPO and lactoferrin proteins were expressed in rats LG. The house keeping proteins alpha-tubulin and beta actin can be used as loading controls. GPX3 expression was significantly reduced in LG of female compared to male (P<0.05), however LPO was higher in LG of females. Total peroxidase activity was 0.29±0.03 mU/ml in male and 0.98±0.04 mU/ml in female rat lacrimal gland (P=0.0022).
The present work shows that proteins related to innate anti microbial defense have different expressions in LG of male and female rats. Both housekeeping proteins alpha-tubulin and beta actin can be used as loading controls. Those data suggest that innate defense against external agents is more intense in female, however the protective capacity against internal damage is slight higher in male rats. Moreover, it suggests that peroxidase activity, as measured by enzymatic assays express better the secretory function rather than antioxidant capacity.
This PDF is available to Subscribers Only