April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Sex Difference in Innate Anti Microbial and House Keeping Proteins in Rat Lacrimal Gland
Author Affiliations & Notes
  • Monica Alves
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Lilian E. Costa Mendes da Silva
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Stella F. de Freitas
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Ana C. Dias
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Leonardo T. Malki
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Carolina M. Módulo
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Jayter S. de Paula
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Eduardo M. Rocha
    Ophthalmology, University of Sao Paulo, Ribeirão Preto, Brazil
  • Footnotes
    Commercial Relationships  Monica Alves, None; Lilian E. Costa Mendes da Silva, None; Stella F. de Freitas, None; Ana C. Dias, None; Leonardo T. Malki, None; Carolina M. Módulo, None; Jayter S. de Paula, None; Eduardo M. Rocha, None
  • Footnotes
    Support  FAPESP CNPq CAPES FAEPA
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3746. doi:
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      Monica Alves, Lilian E. Costa Mendes da Silva, Stella F. de Freitas, Ana C. Dias, Leonardo T. Malki, Carolina M. Módulo, Jayter S. de Paula, Eduardo M. Rocha; Sex Difference in Innate Anti Microbial and House Keeping Proteins in Rat Lacrimal Gland. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3746.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Several proteins are part of the innate anti microbial defense and several others are being used as endogenous biomarkers of exocrine gland function. However, their sexual differences and the expression of different peroxidases remain unclear . The aims of this study are 1) to compare the expression of house keeping proteins in lacrimal gland of male and female rats 2) to identify and distinguish the members of peroxidase superfamily in rat lacrimal gland (LG) of both genders.

Methods: : Male and female Wistar rats lacrimal glands (LG) were homogenized and evaluated by Western blot (WB) to compare the expression of glutathione peroxidase-3 (GPX3), lactoperoxidase (LPO), lactoferrin and alpha-tubulin and beta actin. Total peroxidase activity was also compared among the groups after protein normalization (bradford method).

Results: : Western blot analysis had shown that GPX3, LPO and lactoferrin proteins were expressed in rats LG. The house keeping proteins alpha-tubulin and beta actin can be used as loading controls. GPX3 expression was significantly reduced in LG of female compared to male (P<0.05), however LPO was higher in LG of females. Total peroxidase activity was 0.29±0.03 mU/ml in male and 0.98±0.04 mU/ml in female rat lacrimal gland (P=0.0022).

Conclusions: : The present work shows that proteins related to innate anti microbial defense have different expressions in LG of male and female rats. Both housekeeping proteins alpha-tubulin and beta actin can be used as loading controls. Those data suggest that innate defense against external agents is more intense in female, however the protective capacity against internal damage is slight higher in male rats. Moreover, it suggests that peroxidase activity, as measured by enzymatic assays express better the secretory function rather than antioxidant capacity.

Keywords: cornea: tears/tear film/dry eye • lacrimal gland • differentiation 
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