April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Lymphocyte-Dependence of the Adenosine A2a Receptor Up-Regulation in the Male NOD Mouse Dry Eye Model
Author Affiliations & Notes
  • J. Peter Gierow
    School of Natural Science, Linnaeus University, Kalmar, Sweden
  • Stina K. Carlsson
    School of Natural Science, Linnaeus University, Kalmar, Sweden
  • Daniel Diaz
    Pharmacology and Pharmaceutical Sciences, University of Southern California School of Pharmacy, Los Angeles, California
  • Kaijin Wu
    Pharmacology and Pharmaceutical Sciences, University of Southern California School of Pharmacy, Los Angeles, California
  • Sarah F. Hamm-Alvarez
    Pharmacology and Pharmaceutical Sciences, University of Southern California School of Pharmacy, Los Angeles, California
  • Footnotes
    Commercial Relationships  J. Peter Gierow, None; Stina K. Carlsson, None; Daniel Diaz, None; Kaijin Wu, None; Sarah F. Hamm-Alvarez, None
  • Footnotes
    Support  Linnaeus University Faculty grant, Magnus Bergvall Foundation (JPG) and NIH Grant EY11386 (SHA).
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3752. doi:
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      J. Peter Gierow, Stina K. Carlsson, Daniel Diaz, Kaijin Wu, Sarah F. Hamm-Alvarez; Lymphocyte-Dependence of the Adenosine A2a Receptor Up-Regulation in the Male NOD Mouse Dry Eye Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3752.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Studies in our laboratory have recently shown that adenosine potentiates the effect of carbachol on lacrimal gland secretion in rabbit through the A2 receptors (Curr. Eye Res. 2010, 35:466-474), and more recently that the adenosine A2a receptor is up-regulated in the male non-obese diabetic (NOD) mouse dry eye model (Invest. Ophthalmol. Vis. Sci. 51, E-abstract: p4139, 2010). In the present study, we have investigated the role of lymphocytes in the up-regulation of A2a receptors using NOD/SCID, a strain essentially lacking functional lymphocytes.

Methods: : Lacrimal glands from 12 week old NOD, NOD/SCID, and BALB/c mice, the latter as a control, were used in this study. To detect adenosine receptor presence on mRNA level quantitative real-time PCR (qPCR) was performed. Immuno-histochemistry of sectioned cryonic lacrimal glands were used to detect protein expression.

Results: : The results revealed that the mRNA expression of the A2a receptor in lacrimal glands was higher in 12 weeks old NOD compared to BALB/c in the qPCR by 20-fold (p<0.001). A comparison between the strains showed that the A2a expression was 10-fold higher in NOD than in NOD/SCID (p<0.01), but no significant differences were obtained between NOD/SCID and BALB/c. Similar patterns were seen in the immuno-histochemistry, where A2a receptor staining was most intense in 12 weeks old NOD mice, and the increased staining was observed on both acinar cells and infiltrating lymphocytes.

Conclusions: : Our results show that the up-regulation of the A2a receptor mRNA expression in the male NOD mice is at least partly lymphocyte-dependent, but that the immuno-staining is localized to both acinar cells and lymphocytes.

Keywords: lacrimal gland • cornea: tears/tear film/dry eye • receptors 
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