Lactoferrin Treatment For Age Related Dry Eye In Mice

Abstract

Purpose: : We previously reported that lacrimal gland secretory function was related to aging-induced dry eye disease in mouse. Lactoferrin, which was main glycoprotein conponent of tear, is a present in human mil multiple functions, including antiinflammatory effects and promotion of cell growth and DNA synthesis, and anti-angiogenic and anti-tumoral properties. We investigated oral lactoferrin effect in age-related lacrimal dysfunction.

Methods: : Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into ad libitum (AL) and oral lactoferrin treatment groups (LF). After 6 months, tear function was examined under conscious state. After euthanasia, lacrimal glands were subjected to histological examination, and assessment of oxidative stress with 8-hydroxy-2 deoxyguanosine (8-OHdG) and 4-hydroxynonenal (HNE) antibodies. Real time PCR was also performed for MCP-1 and TNF-alpha.

Results: : The volume of tear secretion was significantly more in the LF group compared to the AL group. LF reduced the inflammatory cell infiltration, the concentration of 8-OHdG and the extent of staining with HNE in the lacrimal gland, compared to AL. In the real time PCR, MCP-1 and TNF-alpha expression were lower in the LF group compared to AL group.

Conclusions: : LF might attenuate oxidative stress damage and suppress inflammatory mediators in the lacrimal gland and preserved lacrimal gland functions.

Keywords: cornea: tears/tear film/dry eye • aging • lacrimal gland