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Motoko Kawashima, Tetsuya Kawakita, Takaaki Inaba, Mitsuhiro Watanabe, Ken Shinmura, Shigeto Shimmura, Kazuo Tsubota, Kazuo Tsubota; Lactoferrin Treatment For Age Related Dry Eye In Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3767.
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© ARVO (1962-2015); The Authors (2016-present)
We previously reported that lacrimal gland secretory function was related to aging-induced dry eye disease in mouse. Lactoferrin, which was main glycoprotein conponent of tear, is a present in human mil multiple functions, including antiinflammatory effects and promotion of cell growth and DNA synthesis, and anti-angiogenic and anti-tumoral properties. We investigated oral lactoferrin effect in age-related lacrimal dysfunction.
Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into ad libitum (AL) and oral lactoferrin treatment groups (LF). After 6 months, tear function was examined under conscious state. After euthanasia, lacrimal glands were subjected to histological examination, and assessment of oxidative stress with 8-hydroxy-2 deoxyguanosine (8-OHdG) and 4-hydroxynonenal (HNE) antibodies. Real time PCR was also performed for MCP-1 and TNF-alpha.
The volume of tear secretion was significantly more in the LF group compared to the AL group. LF reduced the inflammatory cell infiltration, the concentration of 8-OHdG and the extent of staining with HNE in the lacrimal gland, compared to AL. In the real time PCR, MCP-1 and TNF-alpha expression were lower in the LF group compared to AL group.
LF might attenuate oxidative stress damage and suppress inflammatory mediators in the lacrimal gland and preserved lacrimal gland functions.
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