April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Lactoferrin Treatment For Age Related Dry Eye In Mice
Author Affiliations & Notes
  • Motoko Kawashima
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Tetsuya Kawakita
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Takaaki Inaba
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Mitsuhiro Watanabe
    Department of Internal Medicine,
    Keio University School of Med, Shinjuku ku, Japan
  • Ken Shinmura
    Department of Internal Medicine,
    Keio University School of Med, Shinjuku ku, Japan
  • Shigeto Shimmura
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Kazuo Tsubota
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Kazuo Tsubota
    Department of Ophthalmology,
    Keio University School of Med, Shinjuku ku, Japan
  • Footnotes
    Commercial Relationships  Motoko Kawashima, NRL Pharma Inc. (F); Tetsuya Kawakita, NRL Pharma Inc. (F); Takaaki Inaba, None; Mitsuhiro Watanabe, None; Ken Shinmura, None; Shigeto Shimmura, None; Kazuo Tsubota, None; Kazuo Tsubota, None
  • Footnotes
    Support  NRL Pharma Inc.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3767. doi:
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      Motoko Kawashima, Tetsuya Kawakita, Takaaki Inaba, Mitsuhiro Watanabe, Ken Shinmura, Shigeto Shimmura, Kazuo Tsubota, Kazuo Tsubota; Lactoferrin Treatment For Age Related Dry Eye In Mice. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3767.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We previously reported that lacrimal gland secretory function was related to aging-induced dry eye disease in mouse. Lactoferrin, which was main glycoprotein conponent of tear, is a present in human mil multiple functions, including antiinflammatory effects and promotion of cell growth and DNA synthesis, and anti-angiogenic and anti-tumoral properties. We investigated oral lactoferrin effect in age-related lacrimal dysfunction.

Methods: : Twelve-month-old male C57BL/6Cr Slc mice were randomly divided into ad libitum (AL) and oral lactoferrin treatment groups (LF). After 6 months, tear function was examined under conscious state. After euthanasia, lacrimal glands were subjected to histological examination, and assessment of oxidative stress with 8-hydroxy-2 deoxyguanosine (8-OHdG) and 4-hydroxynonenal (HNE) antibodies. Real time PCR was also performed for MCP-1 and TNF-alpha.

Results: : The volume of tear secretion was significantly more in the LF group compared to the AL group. LF reduced the inflammatory cell infiltration, the concentration of 8-OHdG and the extent of staining with HNE in the lacrimal gland, compared to AL. In the real time PCR, MCP-1 and TNF-alpha expression were lower in the LF group compared to AL group.

Conclusions: : LF might attenuate oxidative stress damage and suppress inflammatory mediators in the lacrimal gland and preserved lacrimal gland functions.

Keywords: cornea: tears/tear film/dry eye • aging • lacrimal gland 
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