April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Mucosal Microvilli In Dry Eye Patients With Chronic Graft-versus-host Disease
Author Affiliations & Notes
  • Yukako Tatematsu
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
    Schepens Eye Research Institute, Boston, Massachusetts
  • Yoko Ogawa
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Shigeto Shinmura
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Murat Dogru
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
    J & J Ocular Surface and Visual Optics Department, Keio University School of Medicine, Tokyo, Japan
  • Toshihiro Nagai
    Department of Pathology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Saori Yaguchi
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Kazuto Yamazaki
    Department of Pathology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Kaori Kameyama
    Department of Pathology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Yutaka Kawakami
    Division of Cellular Signaling, Institute of Advanced Medical Research,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Kazuo Tsubota
    Department of Ophthalmology,
    Keio University School of Medicine, Shinjyuku-ku, Japan
  • Footnotes
    Commercial Relationships  Yukako Tatematsu, None; Yoko Ogawa, None; Shigeto Shinmura, None; Murat Dogru, None; Toshihiro Nagai, None; Saori Yaguchi, None; Kazuto Yamazaki, None; Kaori Kameyama, None; Yutaka Kawakami, None; Kazuo Tsubota, None
  • Footnotes
    Support  the Japanese Ministry of the Education, Science, Sports and Culture, #20592058
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3799. doi:
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      Yukako Tatematsu, Yoko Ogawa, Shigeto Shinmura, Murat Dogru, Toshihiro Nagai, Saori Yaguchi, Kazuto Yamazaki, Kaori Kameyama, Yutaka Kawakami, Kazuo Tsubota; Mucosal Microvilli In Dry Eye Patients With Chronic Graft-versus-host Disease. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3799.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : We investigated the histopathological features of the conjunctival microvilli in patients with chronic graft-versus-host disease (cGVHD).

Methods: : Conjunctival tissue specimens from patients with cGVHD or Sjögren’s syndrome (SS) or from healthy individuals were examined by light and electron microscopy, impression cytology, and immunohistochemistry.

Results: : The cGVHD conjunctivae showed significantly more metaplasia and fewer goblet cells than the SS and normal conjunctivae. Abundant CD8+ T cells infiltrated the basal epithelia in the cGVHD conjunctiva. The microvilli per standard epithelial unit and the secretory vesicles were counted by analyzing electron micrographs. The mean number of mucosal microvilli was significantly lower in the cGVHD than in the SS or normal specimens, and the microvilli were significantly shorter, with a smaller height/width ratio. The mean number of secretory vesicles was also significantly lower, and the membrane-spanning mucin thinner, in the cGVHD compared with the SS and normal specimens.

Conclusions: : The conjunctival mucosal microvilli of cGVHD patients were significantly different in number and morphology from those of SS and normal subjects. These may be key factors affecting the stability of the tear-film layer and its contribution to cGVHD-related dry eye.

Keywords: conjunctiva • pathology: human 
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