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Vijay Khetpal, Sankarathi Balaiya, Kakarla Chalam; Resveratrol Inhibits Proliferation In Hypoxia Treated Choroidal Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4853.
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Resveratrol, a naturally occurring phytoalexin found in grapes and red wine inhibits angiogenesis in carcinoma. Its effects are mediated by members of histone deacetylase (HDAC) family of proteins, sirtuin (SIRT1). Hypoxia is a critical factor in the development of choroidal neovascularization (CNV) associated with age related macular degeneration (AMD). The purpose of our study is to evaluate the effect of resveratrol on proliferation of hypoxic choroidal endothelial cells.
Choroidal endothelial cells (RF/6A) were maintained in a semi-confluent state in an appropriate condition. Dose response analysis of choroidal endothelial cell proliferation to resveratrol was evaluated by exposing the cells to escalating dose ( 2, 4, 8, 12 µg/mL) and cell viability was measured using WST-1 assay. Hypoxia was induced to choroidal endothelial cells using cobalt chloride at a concentration of 400 µM and the effect of resveratrol at 2, 4, 8 and 12 µg/mL on hypoxic cells was further evaluated.
Resvertrol induced a dose dependent increase in cell viability in choroidal endothelial cells. We observed an increase in cell viability at doses of 2, 4, 8, 12 µg/mL of resvertrol: 127.43%, 136.33%, 132.90% and 115.6% respectively compared to control. In hypoxic conditions, cells viability was decreased to 100.40 %, 84.21 %, 82.00 %, 86.52 % at 2,4, 8, 12 ug/ml of resveratrol respectively compared to control.
In normal choroidal endothelial cells resveratrol increases cell viability where as in hypoxic conditions (such as AMD), resveratrol inhibits choroidal endothelial cell proliferation in a dose dependant manner
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