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Ryo Asato, Shigeo Yoshida, Keijiro Ishikawa, Yukio Sassa, Yusuke Saeki, Atsushi Ogura, Kazuho Ikeo, Takashi Gojobori, Toshihiro Kono, Tatsuro Ishibashi, IV; Expressed Sequence Tag Analysis of Epiretinal Membranes from Patients with Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4918.
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© ARVO (1962-2015); The Authors (2016-present)
Epiretinal membranes (ERMs) are associated with proliferative vitreoretinopathy (PVR), and can lead to tractional retinal detachments because of the contractile nature of the membrane. The pathogenesis of ERMs has not been fully determined. The purpose of this study was to generate a profile of genes expressed in human ERMs.
A polymerase chain reaction (PCR)-amplified cDNA library was constructed using the RNAs isolated from ERMs obtained during vitrectomy from patients with PVR. The sequence from the 5' end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). Functional annotation was retrieved from Ensemble database and analyzed by the web-bassed FatiGo and DAVID software.
A total of 2688 ESTs were assembled in 1116 nonredundant clusters. Among these, approximately 80 % matched the human cDNA database. The 1116 clusters were subdivided by functional subsets of genes related to ribosomal activity, extracelluar matrix, cell adhesion, and other functions. MALAT1 represented the most abundant transcripts in the ERMs.
The cDNA library constructed from human ERMs will be a valuable source of information that should facilitate a wide range of studies that can establish the molecular mechanisms underlying the development of ERMs.
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