April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Expressed Sequence Tag Analysis of Epiretinal Membranes from Patients with Proliferative Vitreoretinopathy
Author Affiliations & Notes
  • Ryo Asato
    Department of Ophthalmology, Kyushu University, Fukuoka, Japan
    Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Shigeo Yoshida
    Department of Ophthalmology, Kyushu University, Fukuoka, Japan
  • Keijiro Ishikawa
    Department of Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yukio Sassa
    Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Yusuke Saeki
    Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Atsushi Ogura
    National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan, Mishima, Japan
  • Kazuho Ikeo
    National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan, Mishima, Japan
  • Takashi Gojobori
    National Institute of Genetics, Center for Information Biology and DNA Data Bank of Japan, Mishima, Japan
  • Toshihiro Kono
    Department of Ophthalmology, Fukuoka University Chikushi Hospital, Chikushino-shi, Japan
  • Tatsuro Ishibashi, IV
    Department of Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships  Ryo Asato, None; Shigeo Yoshida, None; Keijiro Ishikawa, None; Yukio Sassa, None; Yusuke Saeki, None; Atsushi Ogura, None; Kazuho Ikeo, None; Takashi Gojobori, None; Toshihiro Kono, None; Tatsuro Ishibashi, IV, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4918. doi:
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      Ryo Asato, Shigeo Yoshida, Keijiro Ishikawa, Yukio Sassa, Yusuke Saeki, Atsushi Ogura, Kazuho Ikeo, Takashi Gojobori, Toshihiro Kono, Tatsuro Ishibashi, IV; Expressed Sequence Tag Analysis of Epiretinal Membranes from Patients with Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4918.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Epiretinal membranes (ERMs) are associated with proliferative vitreoretinopathy (PVR), and can lead to tractional retinal detachments because of the contractile nature of the membrane. The pathogenesis of ERMs has not been fully determined. The purpose of this study was to generate a profile of genes expressed in human ERMs.

Methods: : A polymerase chain reaction (PCR)-amplified cDNA library was constructed using the RNAs isolated from ERMs obtained during vitrectomy from patients with PVR. The sequence from the 5' end was obtained for randomly selected clones and used to generate expressed sequence tags (ESTs). Functional annotation was retrieved from Ensemble database and analyzed by the web-bassed FatiGo and DAVID software.

Results: : A total of 2688 ESTs were assembled in 1116 nonredundant clusters. Among these, approximately 80 % matched the human cDNA database. The 1116 clusters were subdivided by functional subsets of genes related to ribosomal activity, extracelluar matrix, cell adhesion, and other functions. MALAT1 represented the most abundant transcripts in the ERMs.

Conclusions: : The cDNA library constructed from human ERMs will be a valuable source of information that should facilitate a wide range of studies that can establish the molecular mechanisms underlying the development of ERMs.

Keywords: proliferative vitreoretinopathy • gene/expression • gene transfer/gene therapy 
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