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Jiwon Jung, So Yeon Lee, Sung Mo Kang; Effects Of Pdgf-bb And Bfgf On Proliferation And Cytokine Production Of Orbital Fibroblast Of Thyroid-associated Ophthalmopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5102.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effects of PDGF-BB and bFGF on proliferationand cytokine production of orbital fibroblast
Orbital fibroblasts were cultured(5 from eyes of thyroid-associatedophthalmopathy, 5 from normal eyes) and classified into 3 groups: (1) Control group, (2) PDGF-BB stimulation group, (3) bFGFstimulation group. Cytokine production(IL-1ß, IL-2, IL-6,IL-8, RANTES) of each group was measured. Proliferation of orbitalfibroblast was measured in each group using a thymidine analogEdU, which can be incorporated into DNA during active DNA synthesis
Only IL-8 showed a borderline significant increase in group2 than group 1(p=0.085). The other cytokines showed no significantdifference. IL-6 and IL-8 production was increased in group3 but it was not statistically significant. Proliferation oforbital fibroblasts was increased from twice to quadruple ingroup 2, but there was no difference in group 1 and 3
PDGF-BB induced orbital fibroblast proliferation and increasedproduction of IL-8. Further study for the stimulating factorsof orbital fibroblasts with larger sample size is needed andwe may expect the fundamental treatment of thyroid-associatedophthalmopathy
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