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Jin S. Yoon, Soolienah Rhiu, Sung J. Lee, Hyun J. Lee, Soo H. Choi, Eun J. Lee; Anti-adipogenic Effect Of Quercetin In An In Vitro Model Of Graves’ Ophthalmopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5104.
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We investigated the therapeutic effect of quercetin, a natural plant flavonoid, in an in vitro model of Graves’ ophthalmopathy, targeting the pathway of adipogenesis.
Confluent fibroblasts were subjected to differentiation protocol including adipogenic supplements and peroxisome proliferator activator gamma (PPARγ) agonist for 10 days, and/or exposed to exogenous IL-1β (10ng/ml), H2O2 10 µM and cigarette smoke extract (CSE) 2% for the first 3 days. Quercetin was treated during 10 days of differentiation, and at day 10, cells were stained with oil red O, and the expression of PPARγ and CCAAT-enhancer-binding proteins (C/EBP) α, β proteins were determined by western blot.
Quercetin inhibited accumulation of intracytoplasmic lipid droplets stained with oil red O and resulted in decreased expression of PPARγ and C/EBPα, β proteins dose-dependently. Quercetin also inhibited the adipogenesis induced by IL-1β, low dose of H2O2 and CSE, which can mimic condition of orbital inflammation, oxidative stress and cigarette smoking in vivo.
These findings provided a basis for further study of the potential usage of quercetin for the treatment of Graves’ ophthalmopathy, especially the fat-predominant type.
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